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Sample GSM72235

Query DataSets for GSM72235

Status

Public on Mar 01, 2006

Title

Med7 ORF-5

Sample type

genomic

Channel 1

Source name

CHIP IP Med7-5 ORF

Organism

Schizosaccharomyces pombe

Characteristics

IP sample med7-TAP

Extracted molecule

genomic DNA

Label

cy5

Channel 2

Source name

WCE

Organism

Schizosaccharomyces pombe

Characteristics

WCE of Med7-TAP

Extracted molecule

genomic DNA

Label

cy3

Description

IgG beads were used for ChIP of chromatin

extracts from Med7-TAP cells. Cell cultures

were then grown to mid logarithmic growth

phase (5 x106 cells/ml) in rich medium before

fixation and ChIP. The resulting ChIP DNA was

labeled with Cy5. The wCE control ChIP DNA was

labeled with Cy3 and simultaneously hybridized

with the Cy5 labeled DNA to the IGR+ORF

microarrays to produce genome wide mediator

occupancy profile.

Data processing

cy5/cy3

Submission date

Aug 26, 2005

Last update date

Dec 27, 2005

Contact name

Claes M Gustafsson

E-mail(s)

[email protected]

Organization name

Gothenburg University

Department

Department of Medical biochemistry and cell biology

Street address

Medcinaregatan 9A

City

Gothenburg

ZIP/Postal code

405 30

Country

Sweden

Platform ID

GPL2789

Series (1)

GSE3213

Genome-wide occupancy profile of mediator and the srb8-11 module reveals interactions with coding regions.

Data table header descriptions
ID_REF
VALUE	log2 of PRE_VALUE
PRE_VALUE	nomarized IP/WCE Med7 IGR value

Data table
ID_REF	VALUE	PRE_VALUE
76	0.4751	1.39
78	-1.4266	0.372
80	-0.7131	0.61
82	-2.0954	0.234
84	-1.0410	0.486
86	2.6098	6.104
88	-4.9523	0.0323
90		0
91		0
92		0
93		0
94		0
95		0
96		0
97		0
98	-0.1408	0.907
100	0.2714	1.207
102	-0.2126	0.863
104	1.3796	2.602
106	0.6022	1.518

Total number of rows: 6469

Table truncated, full table size 106 Kbytes.

Supplementary data files not provided