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| Status |
Public on Mar 01, 2006 |
| Title |
Genome-wide occupancy profile of mediator and the srb8-11 module reveals interactions with coding regions. |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
|
| Summary |
Genome wide interaction of mediator
Keywords: Genome wide interaction of mediator
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| Overall design |
The Mediator complex is involved in preinitiation complex formation and required for the regulated expression of many genes. Mediator exists in two distinct forms, a free form, which contains the Med12, Med13, CDK8, and CycC subunits (Srb8-11), and a smaller form, which lacks these four subunits and associates with RNA polymerase II to form a holoenzyme. Here we use chromatin immuno-precipitation and DNA microarrays to investigate the genome wide localization of Mediator and the Srb8-11 module in fission yeast. Mediator and the Srb8-11 module display very similar genome-wide binding patterns and the Srb8-11 module remains present also at highly transcribed genes. The occupancy profile of Mediator and the Srb8-11 module is distinct from both Pol II and general transcription factors. Unexpectedly, Mediator binds not only to promoters and upstream activating sequences, but also to the downstream coding regions of many genes. Codon region binding does not correlate significantly with transcription activity, but increased Mediator occupancy can be observed after induction of some highly transcribed genes.
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| Contributor(s) |
Zhu X, Wiren M, Sinha I, Linder T, Ekwall K, Gustafsson C, Rasmussen N, Holmberg S |
| Citation(s) |
16630887 |
| |
| Submission date |
Aug 26, 2005 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Claes M Gustafsson |
| E-mail(s) |
[email protected]
|
| Organization name |
Gothenburg University
|
| Department |
Department of Medical biochemistry and cell biology
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| Street address |
Medcinaregatan 9A
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| City |
Gothenburg |
| ZIP/Postal code |
405 30 |
| Country |
Sweden |
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| Platforms (3) |
| GPL2789 |
KI Schizosaccharomyces pombe 10K IGR+ORF |
| GPL2795 |
KI Schizosaccharomyces pombe 23K tiling array |
| GPL3592 |
Eurogentec S. pombe ORF array (D040E) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA92885 |
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