IgG beads were used for ChIP of chromatin extracts from Med13-TAP cells. Cell cultures were then grown to mid logarithmic growth phase (5 x106 cells/ml) in rich medium before fixation and ChIP. The resulting ChIP DNA was labeled with Cy5. The wCE control ChIP DNA was labeled with Cy3 and imultaneously hybridized with the Cy5 labeled DNA to the IGR+ORF microarrays to produce genome wide mediator occupancy profile.
