|
| Status |
Public on Dec 01, 2014 |
| Title |
chronic saline rep3 |
| Sample type |
RNA |
| |
|
| Source name |
saline-treated
|
| Organism |
Mus musculus |
| Characteristics |
tissue: frontal cortex
strain: 129S6/SvEv
genotype: wild type
age: 8-12 weeks
|
| Treatment protocol |
Mice were chronicaly (21 days) injected with SAHA (20 mg/kg) or vehicle, and sacrificed on day after the last injection
|
| Growth protocol |
Mice were housed at 12 h light/dark cycle at 23C with food and water ad libitum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from mouse frontal cortex using RNeasy lipid tissue mini kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated ST-cDNA were prepared after amplification and fragmentation according to the standard Nugen Appluse WT-Amp ST protocol from 200 ng total RNA (Manual, 2010, Nugen).
|
| |
|
| Hybridization protocol |
2.5 ug of ST-cDNA were made into hyb cocktail and hybridized for 18 hr at 45C on GeneChip Gene ST 1.0 Array according to the Nugen Appluse WT-Amp ST protocol. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 using protocol FS450_0007.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G Scanner.
|
| Description |
Gene expression data from frontal cortex of mice chronically treated with saline
|
| Data processing |
The data were analyzed with Expression Console using Affymetrix default analysis settings and RMA as normalization method. The number was log 2 transformed.
|
| |
|
| Submission date |
May 20, 2011 |
| Last update date |
Dec 01, 2014 |
| Contact name |
Javier Gonzalez-Maeso |
| E-mail(s) |
[email protected]
|
| Organization name |
Mount Sinai School of Medicine
|
| Department |
Psychiatry
|
| Lab |
Maeso lab
|
| Street address |
One Gustave L. Levy Place
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29419 |
Chronic SAHA in mouse frontal cortex |
|
