|
| Status |
Public on Jan 20, 2012 |
| Title |
30do mouse spermatids replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
30do mouse spermatids
|
| Organism |
Mus musculus |
| Characteristics |
background strain: BALB/c
gender: male
age: 30 days old
organ: testes
|
| Treatment protocol |
Balb/c 30do male mice were killed by CO2, testes were exised dy the scissors
|
| Growth protocol |
Balb/c male mice were kept in cages till they reach the age of 30 days old
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Testis tubules from wild type Balb/c mice were placed in 1-2 ml of ice-cold PBS-GL buffer (PBS supplemented with 5.6mM D-glucose, 5.4mM DL-lactate, 0.5mM DTT, 0.2mM PMSF, and protease inhibitor cocktail (Roche)) and dissected with scissors followed by homogenisation with 3-4 strokes in a Dounce homogeniser (pestle type A, cell integrity monitored by microscopy). The cellular mixture was filtered through a 70µm cell strainer to obtain a single cell suspension
|
| Label |
biotin
|
| Label protocol |
200ng of total RNA using the Ambion WT kit
|
| |
|
| Hybridization protocol |
We employed standard Affymetrix hybridisation protocol (45C for 17 hours at 60 rpm)
|
| Scan protocol |
Arrays were scanned using a GCS 3000 7G scanner
|
| Description |
Gene expression data from 30 do mouse spermatids
|
| Data processing |
CEL files were generated Affymetrix GeneChip Command Console (AGCC) software. Further analysis performed in R (see manuscript)
|
| |
|
| Submission date |
Jun 07, 2011 |
| Last update date |
Jan 20, 2012 |
| Contact name |
Rohan Williams |
| E-mail(s) |
[email protected]
|
| Organization name |
ANU
|
| Street address |
Garran Rd ACTON
|
| City |
Canberra |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE29781 |
Expression data from 30do mouse spermatid [Affymetrix] |
| GSE29916 |
Functional studies of a H2A.Bbd-like histone variant in mouse spermatogenesis |
|
