|
| Status |
Public on Jun 30, 2011 |
| Title |
Control 0 hour no tetracycline rep2 |
| Sample type |
RNA |
| |
|
| Source name |
mammary
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 3134
receptor: MGI 7035
source strain: RIII
gender: female
time: 0 hour
treatment: control
tet-off: no tetracycline
transgene: A-fos (AP1 dominant negative) and the tetracycline regulator (tTa)
|
| Biomaterial provider |
Hager;hager-lab
|
| Treatment protocol |
Control, untreated 0 hour with tetracycline (1ug/ml)
|
| Growth protocol |
Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland, California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37ºC incubator with 5% CO2. Cells were transferred to 10% charcoal-dextran-treated, heat-inactivated fetal bovine serum for 48 hrs prior to hormone treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells either vehicle treated or treated with 100 nM hormone (dexamethasone, Dex) for 0h, 2h, 4h or 8h. 3134 and AtT-20 RNA for microarray analysis was prepared via standard manufacturer protocols (Qiagen, Valencia, Ca) using cells resuspended in Trizol reagent (Invitrogen, Carlsbad, Ca)
|
| Label |
biotin
|
| Label protocol |
RNA from untreated and dex treated cells were labeled with biotin-CTP using manufacturer's recommendations (Affymetrix, Santa Clara, Ca)
|
| |
|
| Hybridization protocol |
Hybridizations were carried out according the manufacturer (Affymetrix) protocol.
|
| Scan protocol |
Affymterix Gene Chip Scanner 3000
|
| Description |
mouse mammary adenocacinoma tumor with A-fos stably integrated male. The 3134 cell line was derived by transformation of C127, originally isolated from a mammary adenocacinoma tumor of the RIII mouse. A-fos (AP1 dominant negative) and the tetracycline regulator (tTa) were stably integrated into the 3134 cell line., dexamethasone 0 hour control
Dexamethasone untreated, 0 hour control.
|
| Data processing |
Probe-level CEL files were processed through Affymetrix Expression Console using RMA summarization and median normalization methods.
MoGene-1_0-st-v1.r4.pgf
|
| |
|
| Submission date |
Jun 15, 2011 |
| Last update date |
Jun 30, 2011 |
| Contact name |
John A Stamatoyannopoulos |
| E-mail(s) |
[email protected]
|
| Organization name |
Altius Institute / University of Washington
|
| Department |
Genome Sciences
|
| Lab |
Stamatoyannopoulos
|
| Street address |
2211 Elliott Avenue, 6th Floor
|
| City |
SEATTLE |
| State/province |
WA |
| ZIP/Postal code |
98121 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29983 |
Comparison of gene expression profiles for hormone induction in the presence and absence of AP1 binding. |
|
