|
| Status |
Public on Jun 22, 2011 |
| Title |
Native wt biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
CD8+CD28low regulatory T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: wt
treatment: Freshly isolated cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol from Invitrogen for lysis of cells and phase separation according to the manufacturers instructions. RNA was then purified from the aqueous phase using the Qiagen RNeasy Mini Kit. The protocol for RNA clean up was used according to the manufacturer’s instructions.
|
| Label |
Biotin
|
| Label protocol |
Total RNA was converted to biotinylated sense DNA as per the Affymetrix Genechip Whole Transcript (WT) Sense Target Labeling Assay Manual.
|
| |
|
| Hybridization protocol |
Genechips were hybridised overnight at 45 degrees C for 17 hours in an Affymetrix Hybridisation oven.
|
| Scan protocol |
Genechips were scanned using the Affymetrix GSC3000 scanner.
|
| Description |
Native_1_WT
Gene Expression Data
|
| Data processing |
Data processed with Partek Genomics Suite using RMA background correction and quantile array normalisation.
|
| |
|
| Submission date |
Jun 21, 2011 |
| Last update date |
Jun 22, 2011 |
| Contact name |
Mark B Van der Hoek |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Adelaide
|
| Street address |
North Terrace
|
| City |
Adelaide |
| State/province |
South Australia |
| ZIP/Postal code |
5005 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30129 |
AIRE-deficient CD8+CD28low regulatory T lymphocytes fail to control experimental colitis |
|
