|
| Status |
Public on Jul 08, 2011 |
| Title |
Treated mouse 2 |
| Sample type |
RNA |
| |
|
| Source name |
Treated mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: brain and spinal cord
treatment: MOG-DNA
strain: C56BL/6
|
| Treatment protocol |
Mice were treated with MOG-DNA or pCi at 28 and 14 days alter EAE induction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from brain and spinal cord samples using T9424-TRI Reagent® Sigma
|
| Label |
biotin
|
| Label protocol |
Samples were chemically fragmented and biotinylated using the WT Terminal labeling kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Samples were hybridized using Affymetrix hybridization kit materials.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Chips were processed on an Affymetrix Expression Console. Background adjustment by Robust Multi-Array (RMA) algorithm was applied to probes intensities. Raw probes gene level expression were summarized from the corrected intensities and then quantile normalized.
|
| |
|
| Submission date |
Jul 07, 2011 |
| Last update date |
Jul 08, 2011 |
| Contact name |
Ramil Nurtdinov |
| E-mail(s) |
[email protected]
|
| Organization name |
Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
|
| Department |
Bioinformatics and Genomics
|
| Lab |
Computational Biology of RNA Processing
|
| Street address |
Dr. Aiguader 88
|
| City |
Barcelona |
| ZIP/Postal code |
08003 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30482 |
MOG-encoding DNA vaccines ameliorate EAE and display neuroprotective effects in treated mice summary |
|
