|
| Status |
Public on Sep 04, 2012 |
| Title |
1_ES_RESTwt_a |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cells (ES)
|
| Organism |
Mus musculus |
| Characteristics |
strain: Black Agouti 129
cell type: Embryonic Stem Cells (ES)
genotype/variation: RESTwt
biological replicate: a
|
| Growth protocol |
REST wildtype, REST heterozygous knock-out and REST knock-out embryonic stem cells were differentiated into terminal neurons as previously described (M. Bibel, J. Richter, E. Lacroix, Y. A. Barde, Nat Protoc 2, 1034 (2007)).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using TRIzol (Invitrogen) and Rneasy kit from Qiagen (for RNA cleanup)
|
| Label |
biotin
|
| Label protocol |
RNA was processed according to the GeneChip Whole Transcript (WT) Double-Stranded Target Assay Manual from Affymetrix
|
| |
|
| Hybridization protocol |
hybridization was performed according to standard Affymetrix procedure
|
| Scan protocol |
Scanning was performed with Affymetrix GCC Scan Control v. 3.0.0.1214
|
| Description |
1_ES_RESTwt_a.CEL
|
| Data processing |
probesets were summarized and probeset-level values normalized with rma(target=core) function from R/Bioconductor package oligo (version 1.14.0)
|
| |
|
| Submission date |
Jul 30, 2011 |
| Last update date |
Sep 04, 2012 |
| Contact name |
Dirk Schuebeler |
| Organization name |
Friedrich Miescher Institute for Biomedical Research
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE27114 |
Expression data from REST knock-out versus REST wild type cells during in vitro neurogenesis |
| GSE27341 |
Role of REST during neuronal differentiation |
|
