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Status
Public on Aug 11, 2011
Title
BEC.SLN#5
Sample type
RNA
Source name
cutaneous LN
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Dissection and enzymatic digestion, AutoMACS enrichment of CD45- cells, Aria sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
BEC.SLN#5, BECs from the SLN
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Aug 10, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
14.7405
10344622
231.3536
10344624
594.0837
10344633
603.1994
10344637
276.5952
10344653
12.1275
10344658
136.2976
10344674
8.9605
10344679
37.7599
10344707
248.8287
10344713
205.5704
10344723
140.958
10344725
46.0365
10344741
1377.9279
10344743
27.895
10344750
294.2826
10344772
22.0413
10344789
119.3612
10344797
289.9748
10344799
169.4565
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM777040_EA07068_105321_MoGene_BEC.SLN_5.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
