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Status
Public on Aug 11, 2011
Title
Fi.Sk#2
Sample type
RNA
Source name
Skin
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Removal of ears, Cutting of tissue with razor blades, Enzymatic digestion, Aria sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
Fi.Sk#2, gp38+CD31-CD140a+CD45- cells from the ears
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Aug 10, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.6224
10344622
210.0199
10344624
379.0286
10344633
744.6859
10344637
235.8491
10344653
12.9422
10344658
210.1898
10344674
8.8258
10344679
33.1842
10344707
384.7975
10344713
382.0389
10344723
159.5239
10344725
45.931
10344741
1362.1832
10344743
21.9311
10344750
101.1064
10344772
26.7212
10344789
101.8649
10344797
233.4916
10344799
120.145
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM777047_EA07068_110673_MoGene_FI.SK_2.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
