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Status
Public on Aug 11, 2011
Title
FRC.SLN#5
Sample type
RNA
Source name
cutaneous LN
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Dissection and enzymatic digestion, AutoMACS enrichment of CD45- cells, Aria sorting
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
FRC.SLN#5, FRCs from the SLN
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Aug 10, 2011
Last update date
May 24, 2016
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.5423
10344622
282.0598
10344624
398.8662
10344633
785.1337
10344637
208.3611
10344653
14.7046
10344658
91.6909
10344674
9.4983
10344679
40.8356
10344707
131.0363
10344713
473.4899
10344723
408.1276
10344725
150.1289
10344741
1296.3277
10344743
22.6909
10344750
110.2876
10344772
28.2931
10344789
77.0106
10344797
338.345
10344799
104.4662
24922 423 Kbytes .Supplementary file Size Download File type/resource GSM777057_EA07068_105302_MoGene_FRC.SLN_5.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
