|
| Status |
Public on Aug 11, 2011 |
| Title |
St.31-38-44-.SLN#2 |
| Sample type |
RNA |
| |
|
| Source name |
cutaneous LN
|
| Organism |
Mus musculus |
| Characteristics |
age: 6 weeks
exclusion markers: CD45 gp38 CD31 CD44
genetic background: C57BL/6J
phenotype markers: CD45- gp38- CD31- CD44-
separation method: dissection/trimming
Sex: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Enzymatic digestion, MACS CD45 depletion sorting
|
| Label |
Biotin
|
| Label protocol |
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
|
| |
|
| Hybridization protocol |
Affy 1.0 ST Hyb Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
St.31-38-44-.SLN#2, cutaneous lymph node CD44- double negative stromal cell
|
| Data processing |
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Aug 10, 2011 |
| Last update date |
May 24, 2016 |
| Contact name |
Liang Yang |
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
|
