|
| Status |
Public on Aug 15, 2012 |
| Title |
wild-type 1 cardiomyocytes |
| Sample type |
RNA |
| |
|
| Source name |
cardiomyocytes from WT mouse
|
| Organism |
Mus musculus |
| Characteristics |
tissue: ventricular cardiomyocytes
gender: male
strain: C57BL/6
genotype: WT
age: 3 months
|
| Treatment protocol |
No treatments were applied to the animals or tissue.
|
| Growth protocol |
WT and VAChT KD mice were aged to 3 months of age before ventricular cardiomyocytes were excised for RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Fatty and Fibrous Tissue RNA Extraction Kit (Bio-rad).
|
| Label |
biotin
|
| Label protocol |
Biotin end labeled single strand cDNA was prepared according to the Ambion Whole Transcript (WT) Sense Target Labeling Assay Manual and Affymetrix GeneChip WT Terminal Labeling and Hybridization User Manual
|
| |
|
| Hybridization protocol |
5.5 ug of single stranded cDNA was hybridized for 16 hr at 45C on GeneChip Mouse Gene 1.0 ST Arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
gene expression data from cardiomyocytes
|
| Data processing |
The data were analyzed with Partek Genomcis Suite using the RMA normalization method
|
| |
|
| Submission date |
Aug 15, 2011 |
| Last update date |
Aug 15, 2012 |
| Contact name |
David E Carter |
| E-mail(s) |
[email protected]
|
| Organization name |
Robarts Research Institute
|
| Department |
London Regional Genomics Centre
|
| Street address |
1151 Richmond Street North
|
| City |
London |
| State/province |
ON |
| ZIP/Postal code |
N6A5B7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE31395 |
Expression data from WT and VAChT KDHOM whole hearts |
|
