|
Status |
Public on Aug 19, 2011 |
Title |
ESC_2i_rep3 |
Sample type |
RNA |
|
|
Source name |
Embryonic stem cells maintained in 2i condition
|
Organism |
Mus musculus |
Characteristics |
tissue: Mouse embryonic stem cell strain: 129
|
Treatment protocol |
CHIR99021 (3 µM), PD0325901 (1 µM), XAV939 (Sigma, 2 µM), FGF2 (10ng/ml) , activin (10ng/ml)
|
Growth protocol |
GMEM medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Hyclone), 2 mM L-glutamine (Gibco), 1 mM sodium pyruvate (Gibco), 1% nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with the RNeasy Mini Kit (Qiagen).
|
Label |
biotin
|
Label protocol |
Performed by UCLA DNA Microarray core facility
|
|
|
Hybridization protocol |
Performed by UCLA DNA Microarray core facility
|
Scan protocol |
Performed by UCLA DNA Microarray core facility
|
Description |
155739-155730
|
Data processing |
The data analysis was performed using Partek Microarray Software with RMA background correction and quantile normalization.
|
|
|
Submission date |
Aug 18, 2011 |
Last update date |
Aug 19, 2011 |
Contact name |
Qilong Ying |
Organization name |
USC
|
Lab |
Qilong Ying
|
Street address |
1425 san pablo st BCC509b
|
City |
los angeles |
State/province |
CA |
ZIP/Postal code |
90033 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (1) |
GSE31461 |
Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin |
|