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Sample GSM781877 Query DataSets for GSM781877
Status Public on Aug 19, 2011
Title ESC_2i_rep3
Sample type RNA
 
Source name Embryonic stem cells maintained in 2i condition
Organism Mus musculus
Characteristics tissue: Mouse embryonic stem cell
strain: 129
Treatment protocol CHIR99021 (3 µM), PD0325901 (1 µM), XAV939 (Sigma, 2 µM), FGF2 (10ng/ml) , activin (10ng/ml)
Growth protocol GMEM medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Hyclone), 2 mM L-glutamine (Gibco), 1 mM sodium pyruvate (Gibco), 1% nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with the RNeasy Mini Kit (Qiagen).
Label biotin
Label protocol Performed by UCLA DNA Microarray core facility
 
Hybridization protocol Performed by UCLA DNA Microarray core facility
Scan protocol Performed by UCLA DNA Microarray core facility
Description 155739-155730
Data processing The data analysis was performed using Partek Microarray Software with RMA background correction and quantile normalization.
 
Submission date Aug 18, 2011
Last update date Aug 19, 2011
Contact name Qilong Ying
Organization name USC
Lab Qilong Ying
Street address 1425 san pablo st BCC509b
City los angeles
State/province CA
ZIP/Postal code 90033
Country USA
 
Platform ID GPL6246
Series (1)
GSE31461 Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin

Data table header descriptions
ID_REF
VALUE log2 Partek normalized signal intensity

Data table
ID_REF VALUE
10605884 5.61285
10411593 6.06859
10351644 4.81156
10461553 4.85926
10436128 4.54105
10411595 4.58705
10485979 4.61569
10545940 8.79933
10521515 5.72914
10486081 4.9189
10595900 9.06005
10485982 6.62335
10606001 9.35209
10461558 5.12063
10411609 9.15983
10595892 5.00128
10571467 4.45915
10411611 4.77095
10595895 5.41858
10546047 5.42743

Total number of rows: 35399

Table truncated, full table size 583 Kbytes.




Supplementary file Size Download File type/resource
GSM781877.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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