|
| Status |
Public on Aug 19, 2011 |
| Title |
ESC_2i_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic stem cells maintained in 2i condition
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Mouse embryonic stem cell
strain: 129
|
| Treatment protocol |
CHIR99021 (3 µM), PD0325901 (1 µM), XAV939 (Sigma, 2 µM), FGF2 (10ng/ml) , activin (10ng/ml)
|
| Growth protocol |
GMEM medium (Sigma) supplemented with 10% fetal bovine serum (FBS) (Hyclone), 2 mM L-glutamine (Gibco), 1 mM sodium pyruvate (Gibco), 1% nonessential amino acids (Gibco), 0.1 mM β-mercaptoethanol (Sigma)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the RNeasy Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Performed by UCLA DNA Microarray core facility
|
| |
|
| Hybridization protocol |
Performed by UCLA DNA Microarray core facility
|
| Scan protocol |
Performed by UCLA DNA Microarray core facility
|
| Description |
155739-155730
|
| Data processing |
The data analysis was performed using Partek Microarray Software with RMA background correction and quantile normalization.
|
| |
|
| Submission date |
Aug 18, 2011 |
| Last update date |
Aug 19, 2011 |
| Contact name |
Qilong Ying |
| Organization name |
USC
|
| Lab |
Qilong Ying
|
| Street address |
1425 san pablo st BCC509b
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE31461 |
Global gene expression profile of mouse ESC-2i, EpiSC-CHIR/XAV and EpiSC-FGF2/activin |
|
