|
| Status |
Public on Sep 04, 2011 |
| Title |
P28 primary visual cortex of dark reared mice rep1 |
| Sample type |
RNA |
| |
|
| Source name |
P28 primary visual cortex of dark reared mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6
tissue: primary visual cortex
age: P28
|
| Treatment protocol |
C57BL6 mice were reared in normal light and dark conditions (control) till P28, in complete darkness since birth (DR) till P28, or were grown in normal light/dark conditions from birth till P24 and then subjected to lid suturing of one eye till P28.
|
| Growth protocol |
C57BL6 mice were reared till P28.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Mice were euthanized at P28 and their primary visual cortex areas were excised and subjected to RNA isolation. In the case of MD mice only the contralateral to lid suture primary visual cortex was extracted.
|
| Label |
Cy3
|
| Label protocol |
100ng of total RNA were labeled using the Agilent miRNA labeling system.
|
| |
|
| Hybridization protocol |
Microarrays were hybridized to Agilent murine miRNA arrays overnight at 64 ºC.
|
| Scan protocol |
Microarrays were scanned using an Agilent scanner.
|
| Description |
P28 primary visual cortex of dark reared mice replicate 1
|
| Data processing |
Data in GEO were extracted with Agilent feature extractor 10.1 with no additional processing.
|
| |
|
| Submission date |
Aug 19, 2011 |
| Last update date |
Nov 05, 2012 |
| Contact name |
Nikolaos Mellios |
| E-mail(s) |
[email protected]
|
| Organization name |
MIT
|
| Street address |
43 Vassar Street
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
01532 |
| Country |
USA |
| |
|
| Platform ID |
GPL10384 |
| Series (1) |
| GSE31536 |
Changes in miRNA expression in mouse primary visual cortex following visual deprivation |
|
