|
| Status |
Public on Oct 31, 2023 |
| Title |
wk3 ta-activated ILC2 |
| Sample type |
SRA |
| |
|
| Source name |
Lung derived
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Lung derived
time point: wk3
cell type: innate lymphoid cells type 2
strain: C57BL/6
genotype: WT
treatment: tumour carrying, activated
|
| Treatment protocol |
Activated samples are treated with IL33 and TSLP ex vivo for a week before sending off to sequencing. Non-activated samples are freshly isolated ILC2s.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ILC2s are enriched from lung tissues from mice, enriched with negative selection, followed by FACS with lineage and ILC2 specific markers.
Library construction performed based on manufacters instruction (Chromium Single Cell 3' Reagent Kits User Guide (v3.1)).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
Chromium Single Cell Controller (PN 110211)
10xGenomics
|
| Data processing |
Preprocessing done by Cell Ranger v3.0.2
Gene expression analysis by Seurat V3
Assembly: GRCm38_90
Supplementary files format and content: tsv and mtx
|
| |
|
| Submission date |
Oct 30, 2023 |
| Last update date |
Oct 31, 2023 |
| Contact name |
Wilf Jefferies |
| E-mail(s) |
[email protected]
|
| Organization name |
UBC
|
| Street address |
#301-2185 East Mall
|
| City |
Vancouver |
| ZIP/Postal code |
V6T 1Z4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL21103 |
| Series (1) |
| GSE246502 |
A Diversity of Novel Type-2 Innate Lymphoid Cell Subpopulations Revealed During Tumour Expansion |
|
| Relations |
| BioSample |
SAMN38034100 |
| SRA |
SRX22262339 |
