|
| Status |
Public on Feb 25, 2021 |
| Title |
Esrrb_R_day5_-Dox_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Embryonic Stem Cell
|
| Organism |
Mus musculus |
| Characteristics |
cell line: AINV15 Esrrb Rescue Clone
dox withdrawal: 5 days
|
| Treatment protocol |
Each rescue ES clone was grown in +Dox (1ug/ml) media; Dox withdrawal was performed for 5 days
|
| Growth protocol |
rtTA-expressing ES cells Aniv15 were used for derivation of rescue clones and were maintained on primary mouse embryonic fibroblasts with DMEM media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the GeneChip WT cDNA Synthesis and Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridization protocol
|
| Scan protocol |
Standard Affymetrix scanning protocol
|
| Data processing |
Data was normalized using Robust Multichip Average (RMA) method using the Affymetrix Expression Console software
|
| |
|
| Submission date |
Aug 31, 2011 |
| Last update date |
Feb 25, 2021 |
| Contact name |
Ana Sevilla |
| E-mail(s) |
[email protected]
|
| Phone |
+34 675348398
|
| Organization name |
Universidad de Barcelona
|
| Department |
Departamento de Biologia Celular, Fisiologia e Inmunologia
|
| Lab |
4 Floor.
|
| Street address |
Avda Diagonal 643. Edificio Malagef 4rd Floor, Faculty of Biology
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE31791 |
An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feedback Interactions [gene expression] |
| GSE31842 |
An Esrrb and Nanog Cell Fate Regulatory Module Controlled by Feed Forward Loop Interactions. |
|
