GEO help: Mouse over screen elements for information.
Status
Public on Sep 07, 2011
Title
SC.ST34F.BM#2
Sample type
RNA
Source name
Bone marrow
Organism
Mus musculus Characteristics
age: 9 weeks
Extracted molecule
total RNA
Extraction protocol
Whole Bone Marrow from Legs+Hips+Arms crushed and filtered, Enrich cKit (Milteny CD117 beads) and AutoMax, double sorted
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
SC.ST34F.BM#2, CD34+Flk2- ST-HSC CD34+Flk2-Lin-c-kit+Sca1+ multipotent progenitor
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Sep 06, 2011
Last update date
Nov 12, 2020
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE150158 Reanalyzed by
GSE161390
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.0434
10344622
298.5732
10344624
471.654
10344633
757.9425
10344637
210.6834
10344653
15.0306
10344658
379.0352
10344674
9.0955
10344679
33.2426
10344707
262.3555
10344713
1222.7944
10344723
385.7094
10344725
43.6031
10344741
2925.6851
10344743
20.6965
10344750
581.9262
10344772
26.6937
10344789
282.8477
10344797
267.8352
10344799
220.6148
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM791113_EA07068_130478_MOGENE-1_0-ST-V1_SC.ST34F.BM_2.CEL.gz
4.1 Mb
(ftp) (http) CEL
Processed data included within Sample table
