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Sample GSM791118 Query DataSets for GSM791118
Status Public on Sep 07, 2011
Title SC.CMP.BM.DR#2
Sample type RNA
 
Source name Bone marrow
Organism Mus musculus
Characteristics age: 9 weeks
exclusion markers: PI
genetic background: C57BL/6J
phenotype markers: Lineage- Kit+ Sca1- CD34+ FcgRlow
separation method: dissection/trimming
Sex: male
Extracted molecule total RNA
Extraction protocol Whole Bone Marrow from Legs+Hips+Arms crushed and filtered, Enrich cKit (Milteny CD117 beads) and AutoMax, double sorted
Label Biotin
Label protocol Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
 
Hybridization protocol Affy 1.0 ST Hyb Kit
Scan protocol Affymetrix GeneChip Scanner
Description SC.CMP.BM#2, CMP Lin-IL7R-Sca1-ckit+FcgRloCD34+ common myeloid progenitor
Data processing The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
 
Submission date Sep 06, 2011
Last update date Nov 12, 2020
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platform ID GPL6246
Series (1)
GSE15907 ImmGen Microarray Phase 1
Relations
Reanalyzed by GSE150158
Reanalyzed by GSE161390

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity

Data table
ID_REF VALUE
10344620 24.2534
10344622 463.3624
10344624 338.1679
10344633 387.8407
10344637 308.6434
10344653 35.8972
10344658 337.1105
10344674 23.5205
10344679 46.8077
10344707 312.3364
10344713 1408.0121
10344723 571.3858
10344725 66.1955
10344741 3426.4261
10344743 33.3094
10344750 213.0179
10344772 49.6065
10344789 374.9391
10344797 310.557
10344799 233.7723

Total number of rows: 24922

Table truncated, full table size 425 Kbytes.




Supplementary file Size Download File type/resource
GSM791118_EA07068_130472_MOGENE-1_0-ST-V1_SC.CMP.BM.DR_2.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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