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Status
Public on Sep 07, 2011
Title
SC.CMP.BM.DR#2
Sample type
RNA
Source name
Bone marrow
Organism
Mus musculus
Characteristics
age: 9 weeks exclusion markers: PI genetic background: C57BL/6J phenotype markers: Lineage- Kit+ Sca1- CD34+ FcgRlow separation method: dissection/trimming Sex: male
Extracted molecule
total RNA
Extraction protocol
Whole Bone Marrow from Legs+Hips+Arms crushed and filtered, Enrich cKit (Milteny CD117 beads) and AutoMax, double sorted
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
SC.CMP.BM#2, CMP Lin-IL7R-Sca1-ckit+FcgRloCD34+ common myeloid progenitor
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Sep 06, 2011
Last update date
Nov 12, 2020
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246
Series (1)
Relations
Reanalyzed by
GSE150158
Reanalyzed by
GSE161390
Data table header descriptions
ID_REF
VALUE
RMA normalized signal intensity
Data table
ID_REF
VALUE
10344620
24.2534
10344622
463.3624
10344624
338.1679
10344633
387.8407
10344637
308.6434
10344653
35.8972
10344658
337.1105
10344674
23.5205
10344679
46.8077
10344707
312.3364
10344713
1408.0121
10344723
571.3858
10344725
66.1955
10344741
3426.4261
10344743
33.3094
10344750
213.0179
10344772
49.6065
10344789
374.9391
10344797
310.557
10344799
233.7723
Total number of rows: 24922 Table truncated, full table size 425 Kbytes .Supplementary file
Size
Download
File type/resource
GSM791118_EA07068_130472_MOGENE-1_0-ST-V1_SC.CMP.BM.DR_2.CEL.gz
3.9 Mb
(ftp) (http)
CEL
Processed data included within Sample table