|
| Status |
Public on Oct 03, 2011 |
| Title |
Brain Stem 1 copy biological 1 technical 1 |
| Sample type |
RNA |
| |
|
| Source name |
Brain Stem Dosage 1
|
| Organism |
Mus musculus |
| Characteristics |
mouse id: 101
chromosome_dosage: df/+
brain region: Brain Stem
strain: F1 C57BL/6N: 129Sv
gender: male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The samples were amplified and converted to cDNA using the Whole Transcript Expression kit (Ambion), fragmented and labeled using the Whole Transcript Sense Target Labeling Assay (Affymetrix)
|
| |
|
| Hybridization protocol |
The arrays were hybridized and washed using the FS450 fluidics station (Affymetrix)
|
| Scan protocol |
scanned using the GCS300 7G GeneChip Scanner (Affymetrix)
|
| Description |
Mus Musculus Brain Stem Dosage 1
|
| Data processing |
Data was extracted using Expression Console (Affymetrix) with the RMA sketch workflow for gene level analysis
|
| |
|
| Submission date |
Sep 09, 2011 |
| Last update date |
Oct 03, 2011 |
| Contact name |
Guy Horev |
| Organization name |
Cold Spring Harbor Laboratory
|
| Street address |
1 Bungtown Road
|
| City |
Cold Spring Harbor |
| State/province |
New York |
| ZIP/Postal code |
11743 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE32012 |
Dosage-dependent phenotypes in models of 16p11.2 lesions found in autism |
|
