|
| Status |
Public on Sep 14, 2011 |
| Title |
explant vehicle |
| Sample type |
RNA |
| |
|
| Source name |
pancreatic buds explanted and treated with vehicle
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: wild type
tissue: pancreatic bud
developmental stage: e12.5
|
| Treatment protocol |
RNA was isolated from pancreatic buds of Pdx1-tTA (n=3) and littermate Pdx1-tTA; TET-VEGF (n=3) e12.5 embryos, or from e12.5 wild type pancreatic buds explanted and treated with VEGFR2i (n=5) or vehicle (n=5) for 2 day
|
| Growth protocol |
RNA was isolated from pancreatic buds of Pdx1-tTA (n=3) and littermate Pdx1-tTA; TET-VEGF (n=3) e12.5 embryos, or from e12.5 wild type pancreatic buds explanted and treated with VEGFR2i (n=5) or vehicle (n=5) for 2 day
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For transcriptome analysis, RNA was isolated using QIAGEN RNeasy micro Kit
|
| Label |
biotin
|
| Label protocol |
target preparation was performed according to the standard Affymetrix user manual GeneChip® Whole Transcript (WT) Sense Target Labeling Assay chapter 4 from 300ng total RNA
|
| |
|
| Hybridization protocol |
For washing staining and scanning we used standard fluidic protocol FS450_0007 GeneChip® Expression Wash, Stain and Scan User Manual For Cartridge Arrays
|
| Scan protocol |
For washing staining and scanning we used standard fluidic protocol FS450_0007 GeneChip® Expression Wash, Stain and Scan User Manual For Cartridge Arrays
|
| Description |
pancreatic buds explanted and treated with vehicle (n=5, pooled)
|
| Data processing |
The arrays were RMA normalized using Partek Genomic Suite 6.5. Differentially regulated genes were selected based on p-values and ratios using ttest.
|
| |
|
| Submission date |
Sep 13, 2011 |
| Last update date |
Sep 14, 2011 |
| Contact name |
Yuval Dor |
| E-mail(s) |
[email protected]
|
| Organization name |
Hadassah medical school
|
| Street address |
ein-karem
|
| City |
jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE32098 |
Blood vessels restrain pancreas branching, differentiation and growth |
|
