|
| Status |
Public on Sep 01, 2013 |
| Title |
bone_wild-type_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
bone of newbone mice
|
| Organism |
Mus musculus |
| Characteristics |
tissue: bone
genotype/variation: wild-type
strain: 129sv
|
| Growth protocol |
3 paires of newbone mice radii and ulnae from Tnni2del175k mutant mice and their wild-type littermates were collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions.recoveries of long transcripts.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip Mouse Gene 1.0 ST (Affymetrix) and scanned at the UCLA Microarray Core Facility.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Data processing |
The raw data were processed using SUM software 3.02.
|
| |
|
| Submission date |
Sep 15, 2011 |
| Last update date |
Sep 01, 2013 |
| Contact name |
xiaoquan zhu |
| E-mail(s) |
[email protected]
|
| Organization name |
beijing hospital
|
| Street address |
N01 dahua road dongdan beijing
|
| City |
beijing |
| ZIP/Postal code |
100730 |
| Country |
China |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE32163 |
The study of the differential expression profile in bone tissues between Tnni2del175k mutant mice and their wild-type littermates using mRNA array. |
| GSE32401 |
Tnni2del175k mutant mice and their wild-type littermates using mRNA array and ChIP-seq |
|
