|
| Status |
Public on Feb 27, 2024 |
| Title |
C03 vineyard block spot 14 time point 8 |
| Sample type |
RNA |
| |
|
| Source name |
C03 vineyard block spot 14 time point 8
|
| Organism |
Vitis vinifera |
| Characteristics |
cultivar: Vitis vinifera cv. Cabernet Sauvignon (FPS clone 8 grafted onto 110R rootstock)
spot: 14
time point: 8
phenology: harvest ~ 24 .5 Brix
sampling date: 2017-09-12
|
| Treatment protocol |
The vineyard was dissected in 14 homogenously distributed spots across the block to account for any vine vigor and fruit quality spatial variability. Each spot comprised a square of nine vines (3 adjacent vines x 3 rows).
|
| Growth protocol |
The experiment was conducted in 2017 growing season in a commercial vineyard of approximately 12 hectares of Vitis vinifera cv. Cabernet Sauvignon (FPS clone 8 grafted onto 110R rootstock). The vineyard was planted in 1992 and located in the Alexander Valley American Viticultural Area of California (38°42'50N, 122°55'4W). The vines were trained as a bilateral cordon, established at 1.5 m above the ground, in a split canopy configuration, with the two cordons installed on two separate wires and pointing in opposite directions. Vine and row spacings were 1.8 and 3.35 m respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from ~200 mg frozen powder berry pericarp (pulp plus skin without seeds) using the Spectrum™ Plant Total RNA kit (Sigma-Aldrich, St. Louis, Missouri, USA) as previously described (Fasoli et al., 2012).
|
| Label |
Cy3
|
| Label protocol |
The cDNA synthesis, labeling, hybridization and washing reactions were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
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| |
|
| Hybridization protocol |
The cDNA synthesis, labeling, hybridization and washing reactions were performed according to the Agilent Microarray-Based Gene Expression Analysis Guide (V 6.5).
|
| Scan protocol |
Scanning and Feature Extraction was performed by using an Agilent Scanner following the settings and parameters indicated in the instruction manual.
|
| Description |
gene expression in C03 vineyard block spot 14 time point 8
|
| Data processing |
A data-matrix was prepared selecting from each single sub-array outcome file the gProcessedSignalvalues, which are the raw fluorescence intensities of each probe. The data were normalized on the 75th percentile and a correlation analysis was then conducted to assess the consistency of the biological triplicates. Correlation matrixes were prepared using R software and Pearson's Correlation Coefficient (PCC) as the statistical metric.
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| |
|
| Submission date |
Jan 26, 2024 |
| Last update date |
Feb 28, 2024 |
| Contact name |
Alessandra Amato |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Verona
|
| Street address |
strada le grazie 15
|
| City |
VR |
| ZIP/Postal code |
37134 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22427 |
| Series (1) |
| GSE254304 |
Spatial Variability of Grape Berry Maturation Program at the Molecular Level |
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