|
| Status |
Public on Aug 27, 2024 |
| Title |
KO_4F [IgM] |
| Sample type |
protein |
| |
|
| Source name |
Serum
|
| Organism |
Mus musculus |
| Characteristics |
isotype: IgM
gender: Female
genotype: Tlr7-/-
age (month): 6
slide: 2
block: 1
|
| Extracted molecule |
protein |
| Extraction protocol |
Serum samples from recipients collected as per routine protocol as defined above were procured from histocompatibility lab after IRB approval. Sera of all subjects were aliquoted and stored at -80ºC.
|
| Label |
cy5
|
| Label protocol |
8 positive controls (4 mouse IgG/IgM controls with different dilutions and 4 human IgG /IgM control with different dilutions) were also imprinted on the arrays as positive controls.
Mouse serum samples were first treated with DNAse I to remove free-DNA and then applied onto antoantigen arrays with 1:50 dilution. The autoantibodies binding to the antigens on the array were detected with cy3-labeled anti-human IgG and the array slides were scanned with Genepix 4400A scanner with laser wavelengths 532nm for cy3 and 635nm for cy5 to generate Tiff images. Genepix Pro 7.0 software is used to analyze the image and generate the Genepix report (GPR) files (Molecular Devices, Sunnyvale, California, USA). The net fluorescent intensity (NFI) of each antigen was generated by subtracting the local background and negative control (Phosphate buffered saline or simplified as PBS) signal.
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
not provided
|
| Description |
Slide2_635.gpr
|
| Data processing |
NFI were normalized by robust linear model using build-in Ig control with various dilutions
|
| |
|
| Submission date |
Feb 06, 2024 |
| Last update date |
Aug 27, 2024 |
| Contact name |
Chengsong Zhu |
| E-mail(s) |
[email protected]
|
| Phone |
2146455160
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Immunology
|
| Lab |
Microarray & Immune Phenotyping Core
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL34168 |
| Series (1) |
| GSE255215 |
Tlr7 mediates tissue-specific disease manifestations in primary Sjogren’s disease in a sex-biased manner |
|
