|
| Status |
Public on Nov 02, 2011 |
| Title |
WFS1 WT USHT_05_318_ko |
| Sample type |
RNA |
| |
|
| Source name |
hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: 129SV
genotype: WFS1 WT
tissue: hypothalamus
|
| Treatment protocol |
Hypothalami were dissected under dissection microscope
|
| Growth protocol |
Frozen in liquid nitrogen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100 ng Total RNA Labeling protocol, whole transcript sense target labeling assay
|
| |
|
| Hybridization protocol |
25 ng/ul fragmented and labeled DNA target was hybridized according to standard protocols
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000.
|
| Description |
Gene expression data from hypothalamus of wfs1 deficient mice
|
| Data processing |
Images were processed using the Affymetrix Microarray Suite 5.0, cel files were normalized with RMA and further analyzed using Bioconductir software based on R language
|
| |
|
| Submission date |
Nov 01, 2011 |
| Last update date |
Nov 02, 2011 |
| Contact name |
Sulev Koks |
| E-mail(s) |
[email protected]
|
| Phone |
+61864570313
|
| Organization name |
Murdoch University
|
| Department |
Centre for Molecular Medicine and Innovative Therapeutics
|
| Street address |
90 South Street
|
| City |
Perth |
| State/province |
Western Australia |
| ZIP/Postal code |
6150 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE33372 |
Hypothalamic gene expression profile indicates a reduction in G-protein signaling in the wfs1 mutant mice |
|
