gender: Male strain: Bl6J age: 10-12 weeks tissue: epididymal WAT treatment: control time point: 1
Treatment protocol
SR mice were kept awake using 'gentle handing' method from ZT0 until ZT6 for 5 consecutive days, left to sleep ad libitum for 2 days, and sleep restrictiong was repeated for another 5 days. Control mice were kepts undisturbed in parallel.
Extracted molecule
total RNA
Extraction protocol
Total RNA was isolated using Trizol Reagent (Invitrogen)
Label
biotin
Label protocol
Labelling was with GeneChip WT Terminal Labeling Kit (Affymetrix)
Hybridization protocol
17 hours, 60 rpm, 45C
Scan protocol
GeneChip Scanner 3000
Description
fat_Probe50_(MoGene-1_0-st-v1).CEL
Data processing
Data was extracted from CEL files using dChip. Values are MM/PM normalized signal estimates from dChip software.
