|
| Status |
Public on Jan 24, 2012 |
| Title |
pancreas normal, Kras-Pdx1-cre-IKK2, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
pancreas, Pdx1-cre;KrasLSL-G12D;IKK2/b, normal
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B6
genotype: Pdx1-cre;KrasLSL-G12D;IKK2/b
age: 6-8 months
gender: male
tissue: pancreas
|
| Treatment protocol |
Pancreas was directly taken out after the mice were sacrificed.
|
| Growth protocol |
The mice were maintained at the conventional conditions at the animal core facility at MD Anderson Cancer Center.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from pancreas was isolated by Trizol.
|
| Label |
Biotin
|
| Label protocol |
cDNA were labeled according to the Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization was according to the Affymetrx protocol.
|
| Scan protocol |
The images were scanned using the Hewlett-Packard Gene Array scanner G2500A.
|
| Description |
12_Kras-Pdx1-cre-IKK2-N-3
Gene expression data; Pdx1-cre;KrasLSL-G12D;IKK2/b 3
|
| Data processing |
The data were normalized using RMA and analyzed by using Significance Analysis of Microarray (SAM).
|
| |
|
| Submission date |
Nov 08, 2011 |
| Last update date |
Jan 24, 2012 |
| Contact name |
Paul J Chiao |
| E-mail(s) |
[email protected]
|
| Phone |
(713) 794-1030
|
| Fax |
(713) 794-4830
|
| Organization name |
The University of Texas M. D. Anderson Cancer Center
|
| Department |
Department of Molecular and Cellular Oncology
|
| Street address |
1515 Holcombe Blvd
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
770300 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE33322 |
Gene expression analysis between the pancreatic tissues of Pdx1-cre;Kras LSL-G12D and Pdx-cre;KrasLSL-G12D;IKK2/beta F/F mice |
| GSE33323 |
Kras-induced ikk2/nf-kappaB activation by IL-1 alpha and p62 freedforward loops is required for development of pancreatic ductal adenocarcinoma |
|
