|
| Status |
Public on Nov 23, 2011 |
| Title |
KO_IgA6r |
| Sample type |
RNA |
| |
|
| Source name |
KO_IgA
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
genotype/variation: IgA KO (lacking IgA)
strain: balbc
array name: IgA6r
tissue type: jejunum
additional id: 5249(0)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
tissues were homogenized in Trizol or RLT buffer from Qiagen RNAeasy KIT. Total RNA were isolated according to standard protocol
|
| Label |
Cy3
|
| Label protocol |
20 ug of total RNA were directly fluorescently labeled in a reverse transcription reaction using Cy3-dUTP or Cy5-dUTP (GE Healthcare) and Oligo(dT)12-18 Primer and SuperScript II reverse transcriptase
|
| |
|
| Hybridization protocol |
Corresponding experimental (knock-out) and control samples were co-hybridized using MAUI hybridization system as described by Schaupp et al. 2005; PMID: 15679093
|
| Scan protocol |
Images were scanned by GenePix4000B Scanner (Axon Instruments/Molecular Devices).
|
| Description |
IgA6r green
full-thickness 2 cm of jejunum from the middle of small intestine
|
| Data processing |
After subtraction of local background, signal intensity values were filtered to remove those lower than 10 and median normalized using BRB Array Tools.
|
| |
|
| Submission date |
Nov 22, 2011 |
| Last update date |
Nov 23, 2011 |
| Contact name |
Natalia Shulzhenko |
| E-mail(s) |
[email protected], [email protected]
|
| Organization name |
NIAID, USA
|
| Department |
Laboratory of Cellular and Molecular Immunology
|
| Lab |
Ghost lab
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL14915 |
| Series (2) |
| GSE23934 |
Crosstalk between B lymphocytes, microbiota and the intestinal epithelium governs immunity versus metabolism in the gut |
| GSE33884 |
The role of immunoglobulin A |
|
