|
Status |
Public on Nov 23, 2011 |
Title |
control_gata4 |
Sample type |
RNA |
|
|
Source name |
control_gata
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: control tissue: jejunum pair: 4 additional id: 4821 c
|
Extracted molecule |
total RNA |
Extraction protocol |
tissues were homogenized in Trizol or RLT buffer from Qiagen RNAeasy KIT. Total RNA were isolated according to standard protocol
|
Label |
Cy5
|
Label protocol |
20 ug of total RNA were directly fluorescently labeled in a reverse transcription reaction using Cy3-dUTP or Cy5-dUTP (GE Healthcare) and Oligo(dT)12-18 Primer and SuperScript II reverse transcriptase
|
|
|
Hybridization protocol |
Corresponding experimental (knock-out) and control samples were co-hybridized using Agilent protocol
|
Scan protocol |
Images were scanned using Agilent scanner
|
Description |
gata4red full-thickness 2 cm of jejunum from the middle of small intestine
|
Data processing |
After subtraction of local background, signal intensity values were filtered to remove those lower than 10 and median normalized using BRB Array Tools.
|
|
|
Submission date |
Nov 22, 2011 |
Last update date |
Nov 23, 2011 |
Contact name |
Natalia Shulzhenko |
E-mail(s) |
[email protected], [email protected]
|
Organization name |
NIAID, USA
|
Department |
Laboratory of Cellular and Molecular Immunology
|
Lab |
Ghost lab
|
Street address |
9000 Rockville Pike
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL14915 |
Series (2) |
GSE23934 |
Crosstalk between B lymphocytes, microbiota and the intestinal epithelium governs immunity versus metabolism in the gut |
GSE33885 |
The role of transcription factor Gata4 |
|