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Sample GSM838366 Query DataSets for GSM838366
Status Public on Nov 23, 2011
Title control_gata4
Sample type RNA
 
Source name control_gata
Organism Mus musculus
Characteristics genotype/variation: control
tissue: jejunum
pair: 4
additional id: 4821 c
Extracted molecule total RNA
Extraction protocol tissues were homogenized in Trizol or RLT buffer from Qiagen RNAeasy KIT. Total RNA were isolated according to standard protocol
Label Cy5
Label protocol 20 ug of total RNA were directly fluorescently labeled in a reverse transcription reaction using Cy3-dUTP or Cy5-dUTP (GE Healthcare) and Oligo(dT)12-18 Primer and SuperScript II reverse transcriptase
 
Hybridization protocol Corresponding experimental (knock-out) and control samples were co-hybridized using Agilent protocol
Scan protocol Images were scanned using Agilent scanner
Description gata4red
full-thickness 2 cm of jejunum from the middle of small intestine
Data processing After subtraction of local background, signal intensity values were filtered to remove those lower than 10 and median normalized using BRB Array Tools.
 
Submission date Nov 22, 2011
Last update date Nov 23, 2011
Contact name Natalia Shulzhenko
E-mail(s) [email protected], [email protected]
Organization name NIAID, USA
Department Laboratory of Cellular and Molecular Immunology
Lab Ghost lab
Street address 9000 Rockville Pike
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL14915
Series (2)
GSE23934 Crosstalk between B lymphocytes, microbiota and the intestinal epithelium governs immunity versus metabolism in the gut
GSE33885 The role of transcription factor Gata4

Data table header descriptions
ID_REF
VALUE log2 median-normalized signal intensities

Data table
ID_REF VALUE
GE_BrightCorner 6.994618416
DarkCorner 4.857981205
M400003073 14.14282513
M300016566 4.169925213
M400004115 7.108524323
M400008099 6.108524323
M400017004
M200006064 9.647458076
M300008250 8.179908752
M400013727 4.643856049
M400003523
M400006864 3.523561954
M400011153 4.321928024
M200014124 5.554588795
M300009874 9.292321205
M400017476 8.631176949
M400008256 5.754887581
M400006186 7.906890392
M200015372 4.584962368
M200000255 9.14210701

Total number of rows: 35958

Table truncated, full table size 671 Kbytes.




Supplementary file Size Download File type/resource
GSM838366_gata4red.txt.gz 285.2 Kb (ftp)(http) TXT
Processed data included within Sample table

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