|
| Status |
Public on Sep 03, 2024 |
| Title |
Renal cortex (GR KO, healthy), replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse renal cortex (healthy mouse, genotype GR KO)
|
| Organism |
Mus musculus |
| Characteristics |
genotype: tubular epithelial-specific glucocorticoid receptor deficient mice (Pax8-Cre;nr3c1 flox/flox)
acute kidney injury: no
|
| Treatment protocol |
Control and GR-deficient mice were intramuscularly injected with glycerol. A second cohort of mice was not injected (healthy mice). After 24 hours, the renal cortices were dissected from the kidneys.
|
| Growth protocol |
Renal cortices for microarray analysis were freshly isolated from mouse kidneys and directly subjected to RNA isolation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from renal cortices using RNeasy Micro Kit (Qiagen) and amplified using Nugen Ovation One-Direct System (Nugen Technologies Inc.).
|
| Label |
biotin
|
| Label protocol |
cDNA was fragmented and biotin-labeled using Nugen Encore Biotin Module (Nugen Technologies Inc.).
|
| |
|
| Hybridization protocol |
Affymetrix
|
| Scan protocol |
Affymetrix
|
| Description |
Gene expression data from mouse renal cortex under homeostatic conditions (GR KO)
|
| Data processing |
The data (CEL files) were background corrected, normalized and summarized using the rma function in the affy Bioconductor package and annotated on a transcript level using the getNetAffx function from the oligo package. Additional annotation was derived with the mogene10sttranscriptcluster.db package.
|
| |
|
| Submission date |
Aug 29, 2024 |
| Last update date |
Sep 03, 2024 |
| Contact name |
Thomas Hielscher |
| E-mail(s) |
[email protected]
|
| Organization name |
German Cancer Research Center
|
| Department |
Biostatistics
|
| Street address |
INF 280
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE275996 |
Transcriptome of mouse renal cortices without or with acute kidney injury, and without or with renal tubular epithelial cell-specific deficiency of the glucocorticoid receptor |
|
