|
| Status |
Public on Apr 01, 2012 |
| Title |
TAF7 Depletion in Mouse Embryonic Fibroblasts, sample 22 |
| Sample type |
RNA |
| |
|
| Source name |
embryonic fibroblasts, Taf7f/f-1, infected, 24 hrs
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57/BL6 and 129 mix
cell type: mouse embryonic fibroblast (MEF)
genotype: Taf7f/f
mscv cre-gfp retroviral infection: yes
time recovery (hour): 24
|
| Growth protocol |
The MEFs are grown in DMEM with 10% Fetal Calf Serum, Hepes 20mM, and gentamycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA is extracted using shredders and the Qiagen RNeasy mini kit. Their quality was assayed before using for hybridization.
|
| Label |
biotin
|
| Label protocol |
Labeling was performed according to the Affymetrix (Santa Clara, CA) GeneChip Whole Transcript (WT) Sense Target Labelling Assay manual.
|
| |
|
| Hybridization protocol |
Microarrays were hybridized overnight with 5 ug biotin-labelled ss-DNA, and washed in fluidics station wash protocol: FS450_0001.
|
| Scan protocol |
GeneChip scanner 3000 7G was used.
|
| Description |
m22
Taf7f/f-1 and Taf7f/f-2 represent 2 different MEF cell lines.
|
| Data processing |
Exon array data were analyzed with Affymetrix Expression Console SoftwareTM (version 1.1). The Robust Multi-array Analysis (RMA) algorithm was used for gene intensity analysis. Only genes in the “core” set, which represents RefSeq and full-length GenBank mRNAs, were included in the analysis.
|
| |
|
| Submission date |
Dec 30, 2011 |
| Last update date |
Apr 01, 2012 |
| Contact name |
Dinah Singer |
| E-mail(s) |
[email protected]
|
| Organization name |
National Cancer Institute
|
| Department |
Experimental Immunology Branch
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (2) |
| GSE34793 |
The General Transcription Factor TAF7 is Essential for Embryonic Development but Not Essential for the Survival or Differentiation of Mature T Cells (MEF data) |
| GSE34796 |
The General Transcription Factor TAF7 is Essential for Embryonic Development but Not Essential for the Survival or Differentiation of Mature T Cells |
|
