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Series GSE34793 Query DataSets for GSE34793
Status Public on Apr 01, 2012
Title The General Transcription Factor TAF7 is Essential for Embryonic Development but Not Essential for the Survival or Differentiation of Mature T Cells (MEF data)
Organism Mus musculus
Experiment type Expression profiling by array
Summary TAF7, a component of the TFIID complex that nucleates the assembly of transcription preinitiation complexes, also independently interacts with and regulates the enzymatic activities of other transcription factors, including P-TEFb, TFIIH and CIITA, ensuring an orderly progression in transcription initiation. Since not all TAFs are required in terminally differentiated cells, we examined the essentiality of TAF7 in cells at different developmental stages in vivo. Germ-line disruption of the TAF7 gene is embryonic lethal between 3.5 and 5.5 days post coitus. TAF7-deleted mouse embryonic fibroblasts (MEFs) globally cease transcription and stop proliferating. In contrast, whereas TAF7 is essential for the differentiation and proliferation of immature thymocytes, it is not required for subsequent, proliferation-independent differentiation of lineage committed thymocytes or their egress into the periphery. TAF7 deletion in peripheral CD4+ T cells affects only a small number of transcripts. However, TAF7-deleted T cells are not able to undergo activation and expansion in response to antigenic stimuli. These findings suggest that TAF7 is essential for proliferation but not for proliferation-independent differentiation.
 
Overall design We previously provided evidence that TAF7 is a regulator of the early steps of transcription initiation, that it affects the expression of about 65% of transcripts in 293 kidney cells, and of those, some are TAF1-dependent while others are TAF1-independent. Therefore, we next asked whether TAF7 is dispensable for a subset of embryonic fibroblast genes or whether it is required globally. Both TAF7f/f and TAF7+/+ MEF lines were infected by MSCV Cre-GFP retroviruses, and the infected cells (GFP+) were isolated by FACS at 24, 48 and 72 hours post infection. Uninfected cells from each line were harvested at the same time, collected by FACS and processed simultaneously to be used as controls. The experiments were performed in triplicate. All RNAs were extracted using shredders and the Qiagen RNeasy mini kit and their quality assayed before using for hybridization. Total RNA from each of the triplicates was hybridized on the Affymetrix exon array. All exon array data were analyzed with Affymetrix Expression Console SoftwareTM (version 1.1). The Robust Multi-array Analysis (RMA) algorithm was used for gene intensity analysis. Only genes in the “core” set, which represents RefSeq and full-length GenBank mRNAs, were included in the analysis.
 
Contributor(s) Gegonne A, Singer D
Citation(s) 22411629
Submission date Dec 30, 2011
Last update date Mar 03, 2017
Contact name Dinah Singer
E-mail(s) [email protected]
Organization name National Cancer Institute
Department Experimental Immunology Branch
Street address 9000 Rockville Pike
City Bethesda
State/province Maryland
ZIP/Postal code 20892
Country USA
 
Platforms (1)
GPL6193 [MoEx-1_0-st] Affymetrix Mouse Exon 1.0 ST Array [probe set (exon) version]
Samples (65)
GSM855455 TAF7 Depletion in Mouse Embryonic Fibroblasts, sample 1
GSM855456 TAF7 Depletion in Mouse Embryonic Fibroblasts, sample 2
GSM855457 TAF7 Depletion in Mouse Embryonic Fibroblasts, sample 3
This SubSeries is part of SuperSeries:
GSE34796 The General Transcription Factor TAF7 is Essential for Embryonic Development but Not Essential for the Survival or Differentiation of Mature T Cells
Relations
BioProject PRJNA156207

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE34793_RAW.tar 1.5 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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