|
| Status |
Public on Oct 01, 2012 |
| Title |
WT BMDCs Pam3CSK4 24h |
| Sample type |
RNA |
| |
|
| Source name |
Bone marrow derived dendritic cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl6
genotype: wild type
agent: Pam3CSK4
time: 24hr
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from BMDCs with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
BMDCs gene expression in response to CpG or Pam3CSK4
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jan 10, 2012 |
| Last update date |
Oct 01, 2012 |
| Contact name |
Vincent Feng-Sheng Shih |
| Organization name |
UCSD
|
| Department |
Chemistry and Biochemistry
|
| Lab |
UCSD Signaling Systems Laboratory
|
| Street address |
UCSD m/c 0375, NSB 3318 9500, Gilman Dr.
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0375 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE34990 |
Control of RelB during dendritic cell activation integrates canonical and non-canonical NFkB pathways |
|
