|
| Status |
Public on Nov 27, 2024 |
| Title |
frontonasal prominence HH15, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
frontonasal prominence
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: frontonasal prominence
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Samples were dissected in cold PBS, and chopped coarsely with a razorblade. Dissociation into single cells was done using 0.25% trypsin in DMEM and incubation for 15 min at 37°. Occasional mechanical shearing by careful pipetting was applied during the incubation time.
Following instructions from 10X Genomics Chromium Next GEM Gene Expression 3'.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Cell Ranger software (10X Genomics)
Assembly: Gallus gallus GRCg6a
Supplementary files format and content: Tab-separated values and matrix files
|
| |
|
| Submission date |
Nov 13, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Patrick Tschopp |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Basel
|
| Department |
Department of Environmental Sciences
|
| Lab |
Laboratory of Regulatory Evolution
|
| Street address |
Vesalgasse 1
|
| City |
Basel |
| ZIP/Postal code |
4051 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL19787 |
| Series (1) |
| GSE281769 |
Distinct Gene Regulatory Dynamics Drive Skeletogenic Cell Fate Convergence During Vertebrate Embryogenesis [scRNA-Seq] |
|
| Relations |
| BioSample |
SAMN44717866 |
| SRA |
SRX26708351 |
