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| Status |
Public on Nov 27, 2024 |
| Title |
8577 NR SHAM |
| Sample type |
SRA |
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| Source name |
heart
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| Organism |
Mus musculus |
| Characteristics |
tissue: heart
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 30 mg of heart tissue using the Qiagen RNeasy kit
RNA sequencing and quality control were performed Beijing Genomics Institute (BGISEQ-500)
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
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| Description |
Library name: 8577
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| Data processing |
Data analysis–RNA sequencing samples were aligned using Tophat (Trapnell et al, 2009, version 2.0.13). Gene-level read counts were quantified using htseq-count (Anders et al, 2015). Genes expressed above 4 transcripts per million in at least 2 samples were used. In differential expression, a gene to have total normalized reads >10 across all samples. DeSeq (Anders and Huber, 2010) was used for differential gene expression analysis.
Assembly: GRCm38
Supplementary files format and content: excel normalized read count
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| Submission date |
Nov 25, 2024 |
| Last update date |
Nov 27, 2024 |
| Contact name |
Matthew Walker |
| E-mail(s) |
[email protected]
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| Organization name |
University of Washington
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| Street address |
850 Republican
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| City |
Seattle |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platform ID |
GPL23479 |
| Series (1) |
| GSE282759 |
Raising NAD+ Level Stimulates Short-Chain Dehydrogenase/Reductase Proteins to Alleviate Heart Failure Independent of Mitochondrial Protein Deacetylation |
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| Relations |
| BioSample |
SAMN45033472 |
| SRA |
SRX26856623 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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