|
| Status |
Public on Jan 01, 2013 |
| Title |
DDIX-9pH + Sh repA |
| Sample type |
RNA |
| |
|
| Source name |
pH induced parasite
|
| Organism |
Toxoplasma gondii |
| Characteristics |
treatment: alkaline
overexpression: DDHA-AP2IX
strain: type III CTG
Stage: in vitro pH induced bradyzoite
drug treatment: 200nM shield-1
|
| Treatment protocol |
pH8.2 and/or shield-1 treatment
|
| Growth protocol |
parasites were grown in T175 flasks in human foreskin fibroblasts
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA easy, double DNAse digestion
|
| Label |
biotin
|
| Label protocol |
Ambion messageAmp premier
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
n/a
|
| Data processing |
Genespring and R/bioconductor
|
| |
|
| Submission date |
Mar 06, 2012 |
| Last update date |
Jan 24, 2013 |
| Contact name |
Olivier Lucas |
| E-mail(s) |
[email protected]
|
| Organization name |
University of South Florida
|
| Street address |
3720 spectrum blvd
|
| City |
Tampa |
| State/province |
FL |
| ZIP/Postal code |
33612 |
| Country |
USA |
| |
|
| Platform ID |
GPL16542 |
| Series (1) |
| GSE36300 |
Effect of AP2IX-9 overexpression on CTG transcriptome upon alkaline treatment |
|
