the cells were incubated for increasing times, up to 48 h, with 0.1, 1 and 10 µM risperidone. Gene expression was assessed by microarray in cells treated with risperidone 10 µM at 6, 24 and 48 h. Each condition was assessed in quadruplicate. Risperidone was resuspended in DMSO. In each experiment, controls were treated with the vehicle (DMSO) only. The vehicle did not exhibit cytotoxic effects at the concentrations used in the experiments (maximum concentration 1% of total volume).
Growth protocol
SK-N-SH cell line was grown at 37ºC in a humidified incubator with 5% of CO2 in MEM supplemented with 10% FBS, 2 mM L-glutamine, sodium pyruvate, 50 U/mL penicillin and 50 µg/mL streptomycin. Cells were seeded at 3x105 cells/well in 6-well culture plates. The cells were allowed to grow for 48 h to reach 95% confluence.
Extracted molecule
total RNA
Extraction protocol
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label
biotin
Label protocol
Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
Hybridization protocol
Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
Scan protocol
Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
Data processing
RMA processing algorithm. Protocol from Kompetenzzentrum für Fluorescente Bioanalytik Microarray Technology (KFB, BioPark Regensburg GmbH, Regensburg, Germany)
