|
| Status |
Public on Mar 27, 2012 |
| Title |
Tg brain, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult Tg mouse whole brain
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57Bl/6J
gender: male
strain background: Glo1 overexpression
age: adult
tissue: whole brain
|
| Treatment protocol |
Mice were all naïve and were not treated
|
| Growth protocol |
Mice were group housed and aged to adulthood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA isolation with TRIzol and clean up with Qiagen RNeasy kit
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
The details for the hyb/wash/stain are in the Affy protocol: http://media.affymetrix.com/support/downloads/manuals/wt_term_label_ambion_user_manual.pdf. Briefly, the hybridization cocktail was made with the Affy Hyb buffer, DMSO, Oligo B2, and 20X eukaryotic controls and hybridized to the gene arrays for 17hrs at 45C. The arrays were then washed and stained with SAPE (Streptavidin Phycoerythrin binds to the biotin) and scanned.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G
|
| Description |
11-1393
|
| Data processing |
Affymetrix Gene Expression Console Software (v 1.1.2)
|
| |
|
| Submission date |
Mar 26, 2012 |
| Last update date |
Mar 27, 2012 |
| Contact name |
Margaret Gail Distler |
| Organization name |
University of Chicago
|
| Department |
Department of Human Genetics
|
| Lab |
Abraham Palmer
|
| Street address |
920 E. 58th St., CLSC 501
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE36819 |
Expression data from BAC transgenic mice overexpressing Glo1 |
|
