|
Status |
Public on Nov 14, 2014 |
Title |
Colorectal cancer tissue sample (FFPE) 84 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
colorectal cancer, CAIRO arm B
|
Organism |
Homo sapiens |
Characteristics |
tissue: colorectal cancer (FFPE) study: CAIRO study arm: B progression-free survival first line (days): 29 progression: yes
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA isolation protocol available via www.vumc.nl/microarrays. Also described in Brosens et al., J Pathol. 2010 (PMID 20593488).
|
Label |
Cy3
|
Label protocol |
DNA labeling protocol according to Enzo Life Sciences.
|
|
|
Channel 2 |
Source name |
normal colorectum mucosa
|
Organism |
Homo sapiens |
Characteristics |
tissue: normal colon mucosa patient: same patient as in ch1 sample type: reference
|
Extracted molecule |
genomic DNA |
Extraction protocol |
DNA isolation protocol available via www.vumc.nl/microarrays. Also described in Brosens et al., J Pathol. 2010 (PMID 20593488).
|
Label |
Cy5
|
Label protocol |
DNA labeling protocol according to Enzo Life Sciences.
|
|
|
|
Hybridization protocol |
Hybridization protocol available via www.agilent.com.
|
Scan protocol |
Slides were scanned using the Agilent microarray scanner, protocol according to Agilent.
|
Description |
CAIRO arm B: capecitabine and irinotecan first line Normal colon mucosa from the same patient as the tumor was used as reference in the aCGH experiments.
|
Data processing |
The profiles were dewaved (van de wiel et al., Bioinformatics. 2009 (PMID 19276148)), median normalized and corrected for tumor cell percentage by using the R package CGHcall (van de Wiel et al., Bioinformatics. 2007 (PMID 17267432)).
|
|
|
Submission date |
Mar 27, 2012 |
Last update date |
Nov 14, 2014 |
Contact name |
Daoud Sie |
E-mail(s) |
[email protected]
|
Phone |
+31 20 4442428
|
Organization name |
Vrije Universiteit Medical Center
|
Department |
Pathology
|
Lab |
Microarray Core Facility
|
Street address |
De Boelelaan 1117
|
City |
Amsterdam |
ZIP/Postal code |
1081 HV |
Country |
Netherlands |
|
|
Platform ID |
GPL8687 |
Series (2) |
GSE36864 |
Genomic landscape of metastatic colorectal cancer |
GSE63216 |
Recurrent breakpoint genes in colorectal cancer |
|
Relations |
Reanalyzed by |
GSM943166 |