|
| Status |
Public on Apr 27, 2012 |
| Title |
S. pneumoniae KCTC 5080T |
| Sample type |
RNA |
| |
|
| Source name |
S. pneumoniae KCTC 5080T
|
| Organism |
Streptococcus pneumoniae |
| Characteristics |
strain: KCTC 5080T
phenotype: normal
|
| Growth protocol |
S. pneumoniae KCTC 5080T, S. pseudopneumoniae CCUG 49455 T, S. mitis KCTC 3556T, and S. oralis KCTC 13048T strains were grown on Brain Heart Infusion (BHI) agar (Difco., Detroit, MI, U.S.A.) at 37°C for 18 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RiboPure Bacteria Kit (Ambion, UK) following manufacturer’s instructions. RNA quality and concentration was determined by analysis with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA).
|
| Label |
Cy3
|
| Label protocol |
cDNA were prepared from 10 ug total RNA using the SuperScript double-stranded cDNA synthesis kit(Invitrogen). Labeling were carried out according to NimbleGen protocols.
|
| |
|
| Hybridization protocol |
Hybridization were carried out according to NimbleGen protocols.
|
| Scan protocol |
Arrays were scanned using the NimbleGen MS 200 Microarray Scanner
|
| Description |
This sample is of type strain S. pneumoniae KCTC 5080T.
|
| Data processing |
Array data export processing and analysis was performed using NimbleScan v2.5 (RMA algorithm) correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.).
|
| |
|
| Submission date |
Apr 24, 2012 |
| Last update date |
Apr 27, 2012 |
| Contact name |
Heekuk Park |
| E-mail(s) |
[email protected]
|
| Phone |
+82 2 822 5685
|
| Fax |
+82 2 822 5685
|
| Organization name |
Chung-Ang University College of Medicine
|
| Department |
Department of Medical Microbiology
|
| Lab |
Microbiology
|
| Street address |
Heukseok-dong
|
| City |
Seoul |
| ZIP/Postal code |
156-756 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL15485 |
| Series (1) |
| GSE37539 |
Expression analysis of strains Streptococcus pneumoniae KCTC 5080T, Streptococcus mitis KCTC 3556T, Streptococcus oralis KCTC 13048T, and Streptococcus pseudopneumoniae CCUG 49455T |
|
