|
| Status |
Public on Sep 30, 2014 |
| Title |
w1118 biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila control eye discs
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: eye discs
developmental stage: third instar larvae
genotype: w1118 Gal4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using miRNAeasy Mini Kit (Qiagen) according to the manufacturer's instructions. Samples were treated using Rnase-free DNAse I (Qiagen).
|
| Label |
biotin
|
| Label protocol |
RNA was reverse transcribed into cDNA and amplified using in vitro transcription to produce biotin-labeled cRNA
|
| |
|
| Hybridization protocol |
Hybridization was done according to standard Affymetrix procedures. Hybridized cRNA was stained with streptavidin phycoerythrin.
|
| Scan protocol |
Scanning carried out using a Gene Array scanner according to standard Affymetrix procedures.
|
| Description |
Gene expression data from w1118 eye discs
|
| Data processing |
Using Flexarray 1.1, data was adjusted, normalized and summarized using the RMA algorithm
|
| |
|
| Submission date |
Jun 03, 2012 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Heather Collins |
| E-mail(s) |
[email protected]
|
| Organization name |
McGill University
|
| Department |
Biology
|
| Lab |
Nam-Sung Moon
|
| Street address |
1205 Docteur Penfield Avenue
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 1B1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE38430 |
Gene expression data comparing rbf1 mutant and PSC overexpressing Drosophila eye imaginal discs |
|
