|
| Status |
Public on Jun 05, 2012 |
| Title |
mESC1 |
| Sample type |
RNA |
| |
|
| Source name |
Mesc_KSR+LIF_rep1
|
| Organism |
Mus musculus |
| Characteristics |
cell line: embryonic stem (ES) cell line
|
| Treatment protocol |
Untreated - cell were grown in standard XEN or mESC maintenance media.
|
| Growth protocol |
Established cXEN and embryo-derived XEN cells were grown in standard XEN. mESCs were grown in standard mESC media (serum and LIF) for 2 days prior to collecting RNA.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by DNase I treatment in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
biotinylated cRNA was prepared using the Illumina Total Prep 96 RNA Amplification kit from Ambion (Life Technologies)
|
| |
|
| Hybridization protocol |
Illumina direct hybridization protocol
|
| Scan protocol |
Illumina bead array reader scanning protocol
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jun 05, 2012 |
| Last update date |
Jun 05, 2012 |
| Contact name |
Isheng Jason Tsai |
| E-mail(s) |
[email protected]
|
| Organization name |
Wellcome Trust Sanger Institute
|
| Lab |
Parasite Genomics
|
| Street address |
Wellcome Trust Genome Campus
|
| City |
Hinxton |
| ZIP/Postal code |
CB10 1SA |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE38477 |
Conversion of mES to cXEN cells |
|
