NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM97327 Query DataSets for GSM97327
Status Public on Oct 09, 2007
Title Liver_ketotic_cow_early postpartum_6724Cy5
Sample type RNA
 
Channel 1
Source name liver, cow, labelled with Cy5
Organism Bos taurus
Characteristics Liver RNA labelled with Cy5
Extracted molecule total RNA
Label Cy5
 
Channel 2
Source name reference standard RNA, labelled with Cy3
Organism Bos taurus
Characteristics RNA pool of bovine tissues labelled with Cy3
Extracted molecule total RNA
Label Cy3
 
 
Description Cow was feed restricted to 50% of feed intake from 4 days postpartum until onset of ketosis, as determined by thorough clinical examination. Liver RNA was cohybridized with RNA from a pool of bovine tissues.
Data processing loess normalization, log2 transformation
 
Submission date Feb 17, 2006
Last update date Oct 08, 2007
Contact name Juan J. Loor
E-mail(s) [email protected]
Phone 217-244-5957
Organization name University of Illinois
Department Animal Sciences
Lab Mammalian NutriPhysioGenomics
Street address 1207 West Gregory Drive
City Urbana
State/province IL
ZIP/Postal code 61801
Country USA
 
Platform ID GPL2853
Series (1)
GSE4304 Nutrition-induced ketosis alters metabolic and signaling gene networks in liver of periparturient dairy cows

Data table header descriptions
ID_REF
VALUE log2 ratio of median defined by CH1/CH2 (not normalized)
CH1_median CH1 (F635) median fluorescence intensity
CH1_MEAN CH1 (F635) mean fluorescence intensity
CH1_SD CH1 (F635) fluorescence intensity standard deviation
CH1_BKD_Median CH1 (F635) background median fluorescence intensity
CH1_BKD_MEAN CH1 (F635) background mean fluorescence intensity
CH1_BKD_SD CH1 (F635) background fluorescence intensity standard deviation
% > CH1_BKD_+2SD % of feature pixels greater than 2 standard deviations over the background
CH2_median CH2 (F635) median fluorescence intensity
CH2_MEAN CH2 (F635) mean fluorescence intensity
CH2_SD CH2 (F635) fluorescence intensity standard deviation
CH2_BKD_Median CH2 (F635) background median fluorescence intensity
CH2_BKD_MEAN CH2 (F635) background mean fluorescence intensity
CH2_BKD_SD CH2 (F635) background fluorescence intensity standard deviation
% > CH2_BKD_+2SD % of feature pixels greater than 2 standard deviations over the background
Ratio of Medians (635/532) Unnormalized ratio of medians defined by CH1/CH2
Ratio of Means (635/532) Unnormalized ratio of means defined by CH1/CH2
AREA Number of feature pixels
BKD_AREA Number of feature background pixels
CH1_Median - CH1_BKD Channel 1 median signal
CH2_Median - CH2_BKD Channel 2 median signal
CH1_Mean - CH1_BKD Channel 1 mean signal
CH2_Mean - CH2_BKD Channel 2 mean signal

Data table
ID_REF VALUE CH1_median CH1_MEAN CH1_SD CH1_BKD_Median CH1_BKD_MEAN CH1_BKD_SD % > CH1_BKD_+2SD CH2_median CH2_MEAN CH2_SD CH2_BKD_Median CH2_BKD_MEAN CH2_BKD_SD % > CH2_BKD_+2SD Ratio of Medians (635/532) Ratio of Means (635/532) AREA BKD_AREA CH1_Median - CH1_BKD CH2_Median - CH2_BKD CH1_Mean - CH1_BKD CH2_Mean - CH2_BKD
1 0 65535 57258 17327 182 208 128 100 65535 53015 19050 186 196 68 100 1 1.08 80 602 65353 65349 57076 52829
2 0 65535 56783 16910 183 202 114 100 65535 51446 20029 180 190 59 100 1 1.104 80 568 65352 65355 56600 51266
3 0.83 152 234 218 168 188 100 15 168 241 196 177 189 60 16 1.778 1.031 80 474 -16 -9 66 64
4 Null 171 252 209 163 173 83 22 167 233 165 180 188 54 17 -0.615 1.679 80 502 8 -13 89 53
5 -0.515 419 474 171 179 195 117 59 525 524 93 182 187 56 100 0.7 0.863 32 236 240 343 295 342
6 0.138 513 535 155 161 184 109 87 490 505 141 170 181 51 100 1.1 1.116 32 236 352 320 374 335
7 -0.153 366 474 514 169 209 326 9 392 559 801 173 206 276 11 0.9 0.79 52 416 197 219 305 386
8 -0.636 282 396 294 161 171 81 40 344 354 107 156 163 49 81 0.644 1.187 32 236 121 188 235 198
9 -0.377 251 319 276 174 188 105 17 271 307 181 171 184 67 36 0.77 1.066 80 494 77 100 145 136
10 -1.961 213 239 103 185 217 130 3 296 291 93 187 205 79 30 0.257 0.519 80 474 28 109 54 104
11 -1.21 416 580 459 170 193 123 50 742 984 596 173 177 45 98 0.432 0.506 52 385 246 569 410 811
12 -0.895 420 636 628 171 201 144 48 636 883 604 173 195 130 82 0.538 0.655 52 393 249 463 465 710
13 -0.881 278 378 354 171 205 157 20 367 424 267 170 195 125 33 0.543 0.815 80 480 107 197 207 254
14 -0.908 262 295 152 149 154 70 43 366 394 106 154 159 40 100 0.533 0.608 32 176 113 212 146 240
15 -0.049 183 197 112 154 170 98 8 198 231 94 168 176 73 21 0.967 0.683 80 538 29 30 43 63
16 -0.312 251 289 197 164 185 113 19 273 305 207 165 173 65 40 0.806 0.893 52 388 87 108 125 140
17 -0.522 1687 1710 1035 159 174 89 92 2360 2303 1363 166 169 38 95 0.696 0.726 80 472 1528 2194 1551 2137
18 -0.303 1560 1841 1387 154 179 118 88 1901 2493 1815 167 182 82 95 0.811 0.725 80 494 1406 1734 1687 2326
19 -0.166 1985 1871 666 151 165 91 98 2215 2320 473 157 168 60 100 0.891 0.795 52 371 1834 2058 1720 2163
20 -0.275 1621 1689 1026 141 155 73 91 1946 1916 1078 155 158 39 96 0.826 0.879 80 462 1480 1791 1548 1761

Total number of rows: 27648

Table truncated, full table size 2628 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap