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Sample GSM973477 Query DataSets for GSM973477
Status Public on Sep 30, 2012
Title OsWRKY28 over-expressing rice leaves 48 h after M.Oryzae infection replicate 2
Sample type RNA
 
Source name OsWRKY28 over-expressing rice leaves infected with M.Oryzae
Organism Oryza sativa
Characteristics genotype/variation: OsWRKY28 over-expression
infection: M. oryzae
tissue: leaf
Treatment protocol WT and OsWRKY28-overexpressing rice plants were infected with M. oryzae strain Ina86-137 or treated with water at the four-leaf stage and then fourth leaves were harvested from three plants for each samples at 48 h after treatments.
Growth protocol Rice plants were grown in a chamber under a 14-h-light (28℃) and 10-h-dark (24℃) cycle in hydroponic culture.
Extracted molecule total RNA
Extraction protocol RNeasy Plant Mini Kit (Qiagen)
Label Cy3
Label protocol Low RNA input Linear Amplification/Labeling Kit (Agilent)
 
Hybridization protocol Agilent protocol G4140-90040
Scan protocol Agilent scanner model G2565
Description OsWRKY28 over-expressing rice leaves infected with M.Oryzae
Biological replicate 2 of 3.
Data processing After data was analyzed by Fearure Extraction software (version 11, Agilent), data normalization was performed using Partek Genomics Suite software (ver. 6.5; Partek software) by quantile normalization method.
 
Submission date Jul 25, 2012
Last update date Sep 30, 2012
Contact name Kazunori Okada
E-mail(s) [email protected]
Phone +81-3-5841-3070
Organization name The University of Tokyo
Department Biotechnology Research Center
Lab Laboratory of Environmental Biochemistry
Street address 1-1-1, Yayoi, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8657
Country Japan
 
Platform ID GPL8852
Series (1)
GSE39635 WRKY type transcription factor OsWRKY28 overexpression effect on rice leaves 48 h after Magnaporthe Oryzae infection

Data table header descriptions
ID_REF
VALUE Normalized signal intensity (log 2)

Data table
ID_REF VALUE
Os01g0100100|COMBINER_EST|CI448596|0 10.6391
Os01g0100200|mRNA|AK059894|CDS+3'UTR 1.99004
Os01g0100400|mRNA|AK101455|CDS+3'UTR 6.08264
Os01g0100500|mRNA|AK067316|CDS+3'UTR 11.8864
Os01g0100600|mRNA|AK121362|CDS+3'UTR 11.0548
Os01g0100700|mRNA|AK059844|CDS+3'UTR 12.4834
Os01g0100700|mRNA|AK121523|CDS+3'UTR 10.9515
Os01g0100800|mRNA|AK122012|CDS+3'UTR 10.0331
Os01g0100900|COMBINER_EST|CI015509|0 14.5476
Os01g0101200|mRNA|AK067866|CDS+3'UTR 11.1888
Os01g0101200|mRNA|AK104517|CDS+3'UTR 14.0678
Os01g0101200|mRNA|AK104625|CDS+3'UTR 14.0809
Os01g0101200|mRNA|AK104752|CDS+3'UTR 14.0799
Os01g0101200|mRNA|AK119457|CDS+3'UTR 6.97832
Os01g0101300|COMBINER_EST|CI016681|6 9.93616
Os01g0101600|mRNA|AK099952|CDS+3'UTR 6.45409
Os01g0101600|mRNA|AK103820|CDS+3'UTR 11.2493
Os01g0101600|mRNA|AK122118|CDS+3'UTR 11.2719
Os01g0101700|COMBINER_EST|CI525185|3 14.0375
Os01g0101800|mRNA|AK103498|CDS+3'UTR 8.51817

Total number of rows: 42477

Table truncated, full table size 1847 Kbytes.




Supplementary file Size Download File type/resource
GSM973477_US90500268_251524114601_S01_GE1_105_Dec08_1_4.txt.gz 7.6 Mb (ftp)(http) TXT
Processed data included within Sample table
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