|
Status |
Public on Oct 09, 2007 |
Title |
Liver_healthy_cow_early postpartum_7105Cy5 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
liver, cow, labelled with Cy5
|
Organism |
Bos taurus |
Characteristics |
Liver RNA
|
Extracted molecule |
total RNA |
Label |
Cy5
|
|
|
Channel 2 |
Source name |
reference standard RNA, labelled with Cy3
|
Organism |
Bos taurus |
Characteristics |
RNA pool of bovine tissues
|
Extracted molecule |
total RNA |
Label |
Cy3
|
|
|
|
Description |
Cow fed ad libitum early postpartum. Liver RNA was cohybridized with RNA from a pool of bovine tissues.
|
Data processing |
loess normalization, log2 transformation
|
|
|
Submission date |
Feb 17, 2006 |
Last update date |
Oct 08, 2007 |
Contact name |
Juan J. Loor |
E-mail(s) |
[email protected]
|
Phone |
217-244-5957
|
Organization name |
University of Illinois
|
Department |
Animal Sciences
|
Lab |
Mammalian NutriPhysioGenomics
|
Street address |
1207 West Gregory Drive
|
City |
Urbana |
State/province |
IL |
ZIP/Postal code |
61801 |
Country |
USA |
|
|
Platform ID |
GPL2853 |
Series (1) |
GSE4304 |
Nutrition-induced ketosis alters metabolic and signaling gene networks in liver of periparturient dairy cows |
|
Data table header descriptions |
ID_REF |
|
VALUE |
log2 ratio of median defined by CH1/CH2 (not normalized) |
CH1_median |
CH1 (F635) median fluorescence intensity |
CH1_MEAN |
CH1 (F635) mean fluorescence intensity |
CH1_SD |
CH1 (F635) fluorescence intensity standard deviation |
CH1_BKD_Median |
CH1 (F635) background median fluorescence intensity |
CH1_BKD_MEAN |
CH1 (F635) background mean fluorescence intensity |
CH1_BKD_SD |
CH1 (F635) background fluorescence intensity standard deviation |
% > CH1_BKD_+2SD |
% of feature pixels greater than 2 standard deviations over the background |
CH2_median |
CH2 (F532) median fluorescence intensity |
CH2_MEAN |
CH2 (F532) mean fluorescence intensity |
CH2_SD |
CH2 (F532) fluorescence intensity standard deviation |
CH2_BKD_Median |
CH2 (F532) background median fluorescence intensity |
CH2_BKD_MEAN |
CH2 (F532) background mean fluorescence intensity |
CH2_BKD_SD |
CH2 (F532) background fluorescence intensity standard deviation |
% > CH2_BKD_+2SD |
% of feature pixels greater than 2 standard deviations over the background |
Ratio of Medians (635/532) |
Unnormalized ratio of medians defined by CH1/CH2 |
Ratio of Means (635/532) |
Unnormalized ratio of means defined by CH1/CH2 |
AREA |
Number of feature pixels |
BKD_AREA |
Number of feature background pixels |
CH1_Median - CH1_BKD |
Channel 1 median signal |
CH2_Median - CH2_BKD |
Channel 2 median signal |
CH1_Mean - CH1_BKD |
Channel 1 mean signal |
CH2_Mean - CH2_BKD |
Channel 2 mean signal |