|
| Status |
Public on Jun 15, 2013 |
| Title |
24h TNF (10ng/mL)+IL17 (200ng/mL) |
| Sample type |
RNA |
| |
|
| Source name |
normal human epidermal melanocyte
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: juvenile foreskin
cell type: normal human epidermal melanocyte
growth condition: cultured in serum free M2 complete Media
treatment: TNF (10ng/mL)+IL-17 (200ng/mL)
time point: 24 hour cytokine treatment
amplification: single round
|
| Treatment protocol |
Once confluent, the medium was supplemented with or without the following cytokines: recombinant human (rh)-IL-17 (R&D System, Minneapolis, MN) of 20ng/ml or 200ng/mL, and TNFa (Sigma-Aldrich, St Louis, MO, 10ng/ml).
|
| Growth protocol |
Cells were expanded in a serum free, PMA free M2 media (Catalog # C-24300, PromoCell) and maintained at 37°C with 5% CO2 in a humidified incubator. Media was changed every 2-3 days; Passages 3 cells were used for this study. For subcultures, melanocytes were harvested using a detachment kit that contains 0.04% trypsin/ 0.03% EDTA (Catalog # C-41200, PromoCell, Germany).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy Kit
|
| Label |
biotin
|
| Label protocol |
Affymetrix single amplification protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Hewlett-Packard GeneArray Scanner G25Time: 0Time: 0A.
|
| Description |
source of melanocytes: Cryopreserved normal human epidermal melanocytes (NHEM) isolated from juvenile foreskin are purchased from PromoCell (Catalog #. C-12402, Heidelberg, Germany).
|
| Data processing |
GCRMA
Normalized Expression values in log-2 scale
|
| |
|
| Submission date |
Aug 27, 2012 |
| Last update date |
Jun 15, 2013 |
| Contact name |
Claire Q.F. Wang |
| E-mail(s) |
[email protected]
|
| Phone |
+1-212-327-7153
|
| Fax |
+1-212-327-8232
|
| Organization name |
Rockefeller University
|
| Lab |
Laboratory for Investigative Dermatology
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE40413 |
IL-17 and TNF synergistically induce growth-associated cytokines in melanocytes while suppressing melanogenesis |
|
