GEO DataSets

(Submitter supplied) Effects of estrogen on global gene expression: identification of novel targets of estrogen action. Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

4 Samples

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(Submitter supplied) Estrogen receptor-{alpha} (ER{alpha}) and its ligand estradiol play critical roles in breast cancer growth and are important therapeutic targets for this disease. Using chromatin immunoprecipitation (ChIP)-on-chip, ligand-bound ER{alpha} was recently found to function as a master transcriptional regulator via binding to many cis-acting sites genome-wide. Here, we used an alternative technology (ChIP cloning) and identified 94 ER{alpha} target loci in breast cancer cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3283

Platform:

GPL570

9 Samples

Download data: CEL

Analysis of MCF-7 breast cancer cells treated with estradiol for 3 or 6 hours. Results combined with ChIP experiments to identify estrogen receptor alpha binding sites and estradiol target genes in breast cancer cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 2 time sets

Platform:

GPL570

Series:

GSE11506

9 Samples

Download data: CEL

(Submitter supplied) Three human ER+ breast cancer cell lines--MCF-7, T47-D, BT-474--grown with or without estradiol (E2). Keywords: Cell Line Comparison

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1549

Platform:

GPL96

12 Samples

Download data: CEL

Expression profiling of estrogen receptor positive breast cancer cell lines treated with estradiol for 24 hours. MCF-7, T47-D, and BT-474 breast cancer cell lines examined. Results identify candidate genes involved in estrogen stimulated breast cancer growth.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent, 3 cell line sets

Platform:

GPL96

Series:

GSE3529

12 Samples

Download data: CEL

(Submitter supplied) Gene expression changes caused by estrogen treatment of breast cancer cells that re-express ERalpha was investigated by infecting ER-negative MDA-MB-231 breast cancer cells for 24 h with recombinant adenovirus encoding full-length human ERalpha (Ad-ERalpha) or control vector (Ad-LacZ), and treating them with 0·01% ethanol (vehicle control) or 10-8 M 17beta-estradiol (E2). After 48 h of treatment, total RNA was isolated and used for transcript profiling on Affymetrix GeneChips. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1326

Platform:

GPL96

12 Samples

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Analysis of the response of estrogen receptor (ER) negative MDA-MB-231 breast cancer cells infected with full-length ER alpha adenoviral constructs to treatment with 17beta-estradiol (E2). Results provide insight into the anti-proliferative effect of E2 on breast cancer cells reexpressing ER.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 2 protocol sets

Platform:

GPL96

Series:

GSE2251

12 Samples

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(Submitter supplied) Alternative promoters (APs) occur in >30% protein-coding genes and contribute to proteome diversity. However, large-scale analyses of AP regulation are lacking, and little is known about their potential physiopathologic significance. To better understand the transcriptomic impact of estrogens, which play a major role in breast cancer, we analyzed gene and AP regulation by estradiol in MCF7 cells using pan-genomic exon arrays. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL5175 GPL5188

32 Samples

Download data: CEL

(Submitter supplied) Compared the effects of Estrogen and its analog Genistein on mouse thymus. Endogenous estrogen were minimized by removal of ovaries. Samples were collected at 4 time points: 0 days, 2 days, 3 days and 6 days from the start of the treatment. Two biological replicates at each time point. Keywords: comparison of hormone and its analog; Time course

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1275

Platform:

GPL339

14 Samples

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Analysis of thymus of ovariectomized C57BL/6 treated with 17beta-estradiol (estrogen; E2) or genistein, an E2 analog. Thymus tissue examined at various intervals up to 6 days after treatment. Provides insight into E2-mediated regulation of thymic development and immune function.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 3 agent, 4 time sets

Platform:

GPL339

Series:

GSE2889

14 Samples

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(Submitter supplied) 17β-HSD1 expression modulates T47D transcript profile in in steroid-deprived medium. The enzyme specifically regulates apoptosis and cancer-related genes in T47D cells cultured in steroid-deprived medium. Genes that primarily involved in Cell cycle progression, such as the Cyclin A2 gene, CCNA2, are generally down-regulated whereas most genes involved in apoptosis and cell death, such as the proapoptotic gene XAF1 and FGF12, are on the contrary up-regulated by 17β-HSD1 knockdown, and 21% of the modulated genes belong to this latter functional category. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

8 Samples

Download data: CEL

(Submitter supplied) We have determined the gene expression profile induced by 17 alpha-ethynyl estradiol (EE) in Ishikawa cells, a human uterine-derived estrogen-sensitive cell line, at various doses (1 pM, 100 pM, 10 nM, and 1 microM) and time points (8, 24, and 48 h). The transcript profiles were compared between treatment groups and controls (vehicle-treated) using high-density oligonucleotide arrays to determine the expression level of approximately 38,500 human genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

75 Samples

Download data: CEL, CHP

(Submitter supplied) To obtain comprehensive information on 17beta-estradiol (E2) sensitivity of genes that are inducible or suppressible by this hormone, we designed a method that determines ligand sensitivities of large numbers of genes using DNA microarray and a set of simple Perl computer scripts implementing the standard metric statistics, and employed it to characterize effects of low (0-100 pM) concentrations of E2 on the transcriptome profile of MCF7/BUS human breast cancer cells, whose E2 dose-dependent growth curve saturated with 100 pM E2. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2323 GDS2324

Platform:

GPL96

34 Samples

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Analysis of breast cancer MCF7/BUS cells treated with 17beta-estradiol (E2) at concentrations up to 100 pM. Results compared to that from an experiment examining the response to hormone starvation (GDS2323). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 5 dose sets

Platform:

GPL96

Series:

GSE4668

25 Samples

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Analysis of breast cancer MCF7/BUS cells starved of estrogen for up to 2 days. Results compared to that from an experiment (GDS2324) examining the response to low concentrations of 17beta-estradiol (E2). Results provide insight into the E2 sensitivity of E2 inducible or suppressible genes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 time sets

Platform:

GPL96

Series:

GSE4668

9 Samples

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(Submitter supplied) Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh). We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along with previously published data, show that genes that are upregulated by estrogen treatment are biased towards association with polysomes.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

18 Samples

Download data: CEL

(Submitter supplied) DNA Microarray construction : Gene expression analyses were carried out using home-made Nylon microarrays using a previously described technology [10441335]. These microarrays were built using as templates cDNA clones provided by the INRA-AGENAE program [16887034]. All these clones were PCR-amplified at the INRA Resources Centre for Animal Genomics (CRB GADIE, Jouy en Josas, France) using primers designed on the plasmid sequences flanking the cDNA inserts (M13RP1 5’- GTGGAATTGTGAGCGGATAAC and M13RP2 5’-GCAAGGCGATTAAGTTGGG). more...

Organism:

Oncorhynchus mykiss

1 Series

40 Samples

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(Submitter supplied) Changes in the expression of 8400 genes were monitored by cDNA microarray analysis during the first 32 h of human breast cancer (BC) ZR-75.1 cell stimulation with a mitogenic dose of 17beta-estradiol, a timing which corresponds to completion of a full mitotic cycle in hormone-stimulated cells. Hierarchical clustering of 344 genes whose expression either increases or decreases significantly in response to estrogen reveals that the gene expression program activated by the hormone in these cells shows 8 main patterns of gene activation/inhibition. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL970

25 Samples

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(Submitter supplied) The breast cancer promoting effects of estrogen and the chemopreventive effects of tamoxifen are thought to be mediated by the estrogen receptor, a ligand-dependent transcription factor. Therefore, comprehensive analysis of gene expression profiles following estrogen or tamoxifen treatment may help us better understand the role estrogen plays in tumorigenesis. We utilized SAGE (Serial Analysis of Gene Expression) technology to identify genes regulated by estrogen and tamoxifen in the ZR75-1 estrogen dependent breast cancer cell line. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

3 Samples

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(Submitter supplied) Uninvolved tissue taken at the time of primary surgical resection of an adenocarcinoma. Uninvolved status determined by surgical margin and gross appearance, not pathology

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

35 Samples

Download data: TXT