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Links from GEO DataSets

Items: 20

1.

Listeria monocytogenes EGD and EGD-e

(Submitter supplied) Transcriptional profile after growth in MM (minimal medium (Premaratne et al. 2001) supplemented with 50 mM Glucose. Note: strain "EGD" has not been deposited at ATCC/NCTC. It is a derivative of the original Murray strain (Murray et al. 1926), obtained from S.H.E. Kaufmann. Strain "EGD-e" has been obtained from H. Hahn (Berlin) via T. Chakraborty (Gießen) and is deposited under ATTC BAA-679 and in the Pasteur culture collection. more...
Organism:
Listeria monocytogenes EGD-e; Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL7062
3 Samples
Download data: TXT
Series
Accession:
GSE12146
ID:
200012146
2.

Modulation of PrfA activity in Listeria monocytogenes upon growth in different culture media

(Submitter supplied) PrfA activity was studied in L. monocytogenes strain EGD and in an isogenic prfA deletion mutant (EGDΔprfA) carrying multiple copies of the wild-type prfA or the mutant prfA* gene (strains EGDΔprfApPrfA and EGDΔprfApPrfA*) after growth in brain heart infusion (BHI), Luria-Bertani broth (LB) or a defined minimal medium (MM) supplemented either with one of the three PTS-carbohydrates, glucose, mannose and cellobiose, or the non-PTS carbon source glycerol. more...
Organism:
Listeria monocytogenes; Listeria monocytogenes EGD-e
Type:
Expression profiling by array
Platforms:
GPL7062 GPL6554
24 Samples
Download data: TXT
Series
Accession:
GSE12151
ID:
200012151
3.

Listeria monocytogenes EGDΔprfApPrfA and EGDΔprfApPrfA* compared to the wild type strain EGD

(Submitter supplied) Transcriptional profile of prfA, the dependent genes and the PTS genes after growth in MM (minimal medium (Premaratne et al. 2001) supplemented with 50 mM glucose, cellobiose or glycerol. Keywords: prfA overexpressing strains
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL6554
12 Samples
Download data: TXT
Series
Accession:
GSE12145
ID:
200012145
4.

Listeria monocytogenes EGD after growth BHI vs. LB vs. MM

(Submitter supplied) Transcriptional profile of prfA, the dependent genes and the PTS genes after growth in BHI (brain-heart-infusion), LB (Luria Bertani broth) and MM (minimal medium (Premaratne et al. 2001) (each supplemented with 50 mM Glucose). Keywords: media comparison
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL6554
9 Samples
Download data: TXT
Series
Accession:
GSE12143
ID:
200012143
5.

Interference of PTS components with PrfA of L.monocytogenes

(Submitter supplied) Analysis of L. monocytogenes ptsH, hprK and ccpA mutants defective in carbon catabolite repression (CCR) control revealed significant alterations in the expression of PrfA-dependent genes. The hprK mutant showed high up-regulation of PrfA-dependent virulence genes upon growth in glucose-containing media whereas expression of these genes was even slightly down-regulated in the ccpA mutant when compared to the wild-type strain. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL4418
10 Samples
Download data
Series
Accession:
GSE6028
ID:
200006028
6.

Glycerol metabolism and PrfA activity in Listeria monocytogenes

(Submitter supplied) Listeria monocytogenes is able to efficiently utilize glycerol as carbon source. In a defined minimal medium the growth rate is similar (during balanced growth) in presence of glycerol as in presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed in the presence of glycerol (compared to glucose and/or cellobiose) high transcriptional upregulation of the known genes involved in glycerol uptake and metabolism (glpFK, glpD). more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platforms:
GPL6847 GPL6846
23 Samples
Download data: CSV
Series
Accession:
GSE11459
ID:
200011459
7.

Interference of PrfA with glucose uptake

(Submitter supplied) Listeria monocytogenes (Lm) strains expressing high levels of the virulence regulator PrfA (mutant PrfA* or wild-type PrfA) show strong growth inhibition in minimal media when supplemented with glucose but not when supplemented with glucose-6-phosphate compared to the isogenic strains expressing low levels of PrfA. A significantly reduced rate of glucose uptake is observed in the PrfA* over-expressing strain growing in LB supplemented with glucose. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL3517
18 Samples
Download data
Series
Accession:
GSE4414
ID:
200004414
8.

Identification of the major PEP-phosphotransferase systems (PTS) for glucose, mannose and cellobiose of L. monocytogenes

(Submitter supplied) Identification of the major PEP-phosphotransferase systems (PTS) for glucose, mannose and cellobiose of Listeria monocytogenes, and their significance for extra- and intracellular growth. Transcriptional profile of prfA, the dependent genes and the PTS genes after growth in MM (minimal medium (Premaratne et al. 2001)) (supplemented with 10 mM Glucose). Listeria monocytogenes EGD-e possesses 86 pts genes encoding 29 complete and several incomplete PEP-dependent phosphotransferase systems (PTS) for the transport of carbohydrates and sugar alcohols. more...
Organism:
Listeria monocytogenes EGD-e; Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL6554
24 Samples
Download data: TXT
Series
Accession:
GSE18363
ID:
200018363
9.

Listeria monocytogenes Sigma B, but not PrfA, shows strain specific contributions to growth under salt stress, and prolongated lag phase of a sigB null mutant across strains

(Submitter supplied) Listeria monocytogenes is well known to have the ability to survive and grow under a variety of stress conditions. The ability to survive and grow under osmotic stress conditions in particular appears to be important for both growth in certain foods and food associated environments as well as for host infection. To characterize the contributions of transcriptional regulators important for stress response and virulence (i.e., Sigma B - σB and PrfA), we initially analyzed three L. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL4281
1 Sample
Download data: GPR
Series
Accession:
GSE49056
ID:
200049056
10.

RNA-sequencing of L. monocytogenes under PrfA-inducing conditions refines the PrfA regulon and reveals a putative novel PrfA-dependent gene and its associated PrfA box

(Submitter supplied) Purpose: The objective of the present study was to refine the PrfA regulatory network using RNA-seq to characterize the L. monocytogenes transcriptome in a wt, ∆prfA, and prfA* under PrfA-inducing conditions. Methods: Sequence reads were aligned to the L. monocytogenes 10403S genome using the BWA-MEM algorithm, version 0.7.3a. The data for coverage per base on the sense and antisense strands were analyzed separately using SAMtools. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26477
9 Samples
Download data: TXT
Series
Accession:
GSE146507
ID:
200146507
11.

regulation of Listeria monocytogenes internalin-like genes by σB and PrfA as revealed by subgenomic microarray analyses

(Submitter supplied) The Listeria monocytogenes genome contains more than 20 genes that encode cell surface-associated proteins termed internalins, which are characterized by the presence of multiple leucine-rich repeats. Subsets of internalin genes have been reported previously as transcriptionally regulated by the stress responsive sigma factor B (inlA, inlB, inlC2 and inlD) and the pleiotropic transcriptional activator PrfA (inlA, inlB, inlC). more...
Organism:
Listeria monocytogenes 10403S; Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL4604
4 Samples
Download data: GPR
Series
Accession:
GSE6421
ID:
200006421
12.

Characterization of the SigB-PrfA regulatory network in Listeria monocytogenes

(Submitter supplied) Listeria monocytogenes SigB and PrfA are pleiotropic regulators of stress response and virulence gene expression, which have been shown to co-regulate genes in L. monocytogenes. We performed whole genome transcriptional profiling in the presence of PrfA* and active SigB, to identify the overlaps between the PrfA virulence regulon and the SigB stress response regulon. In L. monocytogenes, the PrfA* allele contributes to the activation of virulence genes to a level comparable to that of intracellular growing L. more...
Organism:
Listeria innocua; Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL5029
24 Samples
Download data: GPR
Series
Accession:
GSE11347
ID:
200011347
13.

Pyruvate carboxylase and carbon metabolism in Listeria monocytogenes

(Submitter supplied) Transcriptional profile of wild type L. monocytogenes (EGDe) and a pycA mutant strain was compared on growth in BHI. The human pathogen L. monocytogenes is a facultatively intracellular bacterium that survives and replicates in the cytosol of many mammalian cells. The listerial metabolism, especially under intracellular conditions , is still poorly understood. Recent studies analyzed the carbon metabolism of L. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL6846
4 Samples
Download data: CSV
Series
Accession:
GSE19014
ID:
200019014
14.

Transcriptomic profiling of L-rhamnose utilization in Lmo0753 deletion mutant of Listeria monocytogenes EGDe from human foodborne outbreak associated lineage LII

(Submitter supplied) Among the three major genetic lineages of L. monocytogenes (i.e. LI, LII, and LIII), LI and LII are predominantly associated with foodborne listeriosis outbreaks, whereas LIII is rarely implicated in human infections. In a previous study, we identified a Crp/Fnr family transcription factor lmo0753 that was highly specific to outbreak-associated LI and LII but absent from LIII. Lmo0753 shares two conserved functional domains including a DNA-binding domain with the well-characterized master virulence regulator PrfA in L. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17257
4 Samples
Download data: TXT
Series
Accession:
GSE47721
ID:
200047721
15.

Gene expression profiling of a pressure-tolerant Listeria monocytogenes Scott A ctsR deletion mutant

(Submitter supplied) Transcriptional profling of a Listeria monocytogenes under pressure comparing ctsR mutant and wild type
Organism:
Listeria monocytogenes
Type:
Expression profiling by array
Platform:
GPL6489
4 Samples
Download data
Series
Accession:
GSE22819
ID:
200022819
16.

Peroxide stress in Sigma54 mutant and Wt

(Submitter supplied) Analyses of the Wild type and the sigma 54 mutant strain NZ7306 with and without peroxide treatment: A Lactobacillus plantarum strain with a deletion in the alternative sigma factor 54 (?54) encoding gene rpoN, displayed a 100 fold higher sensitivity to peroxide as compared to its parental strain. This feature could be due to ?54-dependent regulation of genes involved in peroxide stress response. However, transcriptome analyses of the wild type and the mutant strain during peroxide exposure did not support such a role for ?54. more...
Organism:
Lactiplantibacillus plantarum; Lactiplantibacillus plantarum WCFS1
Type:
Expression profiling by array
Platform:
GPL6368
10 Samples
Download data
Series
Accession:
GSE11351
ID:
200011351
17.

Chitin attenuates virulence gene expression of Listeria monocytogenes in vitro

(Submitter supplied) External signals are key for bacteria to sense their immediate environment and fine-tune gene expression accordingly. The foodborne pathogen Listeria monocytogenes senses a range of environmental cues in order to activate or deactivate the virulence-inducing transcriptional factor PrfA during transition between infectious and saprophytic lifecycles. Chitin is an abundant biopolymer formed from linked β-(1–4)-N-acetyl-D-glucosamine residues associated with fungi, the exoskeleton of insects and often incorporated into foods as a thickener or stabiliser. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26477
6 Samples
Download data: XLSX
Series
Accession:
GSE154844
ID:
200154844
18.

Transcriptional adaptation of L. monocytogenes EGD to oxygen availability

(Submitter supplied) Comparison of aerobic and anaerobic transcriptome of L. monocytogenes EGD L. monocytogenes EGD cells used in this study were grown either aerobically or anaerobically to an OD600 = 0.70 - 0.75 and the transcriptome of these two conditions was compared
Organism:
Listeria monocytogenes EGD-e; Listeria monocytogenes EGD
Type:
Expression profiling by array
Platform:
GPL17774
12 Samples
Download data: GPR
Series
Accession:
GSE52325
ID:
200052325
19.

The RNA-binding protein PNPase regulates biofilm formation and virulence in Listeria monocytogenes

(Submitter supplied) Biofilm-related diseases are typically persistent infections, and a challenge for medical treatment. Biofilms are communities of bacteria that attach to surfaces and are enclosed in an extracellular matrix. These sessile microorganisms can endure external stresses like nutrient deprivation, antibiotic treatments, and immune defences. Therefore, biofilms create conditions favourable for bacterial pathogenesis. more...
Organism:
Listeria monocytogenes
Type:
Expression profiling by high throughput sequencing
Platform:
GPL32523
4 Samples
Download data: TXT
Series
Accession:
GSE210097
ID:
200210097
20.

Gene Expression Profiling of Listeria monocytogenes in monoculture and in co-culture state in the presence of Bacillus subtilis both as planktonic cells and in biofilms.

(Submitter supplied) To understand the behaviour in terms of special genome components that are expressed by L.monocytogenes in the presence of another bacterium as may be the condition in its natural environments was our objective, for which we have used microarray gene expression at different time intervals of growth from 4hrs to 24 hrs. Expression of L.monocytogenes as co-cultures, both in broth culture state and biofilm state were differentiated to that of 24hrs pure broth culture. more...
Organism:
Listeria monocytogenes J0161
Type:
Expression profiling by array
Platform:
GPL13262
8 Samples
Download data: TXT
Series
Accession:
GSE27936
ID:
200027936
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