GEO DataSets

(Submitter supplied) MucR is one of the few transcriptional regulatory proteins that has been linked to Brucella pathogenesis. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to copare the gene expression properties of wild type and isogenic mucR mutant cells.

Organism:

Brucella abortus

Type:

Expression profiling by array

Platform:

GPL15577

4 Samples

Download data: CEL, CHP

(Submitter supplied) Purpose:To uncover the related mechanisms underlie virulence attenuation of Brucella canis MucR mutant strain. Methods:Three Brucella canis RM6/66 strains and three Brucella canis ΔmucR strains were grown in TSB at 37℃ until the log phase was reached, total RNA was isolated using the TRIzol according to the manufacturer’s instructions.The sequencing library of each RNA sample was prepared by using NEB Next Ultra Directional RNA Library Prep Kit for Illumina as recommended by the manufacturer. more...

Organism:

Brucella canis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28970

6 Samples

Download data: TXT

(Submitter supplied) The ybeY locus is hypothesized to produce an endoribonuclease, that is expressed during late exponential phase growth and regulate transcript titers. We used custom-made Affymetrix B. abortus strain 2308 derived GeneChips to compare the gene expression properties of wild type and isogenic ybeY mutant cells.

Organism:

Brucella abortus

Type:

Expression profiling by array

Platform:

GPL15577

4 Samples

Download data: CEL, CHP

(Submitter supplied) We sequenced the transcriptomes of B. abortus 2308 wild type, rpoE1, and lovhK deletion strains to assess gene expression in response to oxidative stress. The data represent 2 independent biological replicates of each strain.

Organism:

Brucella melitensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18970

6 Samples

Download data: TAB

(Submitter supplied) A whole genome expression comparision between wild type B. melitensis 16M and ΔBM-LOV-HK (ΔBMEII0679) B. melitensis strains grown in broth culture to determine genes directly and indirectly regulated by BM-LOV-HK.

Organism:

Brucella melitensis bv. 1 str. 16M; Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL16337

6 Samples

Download data: CALLS, PAIR

(Submitter supplied) A mini-microarray containing spotted oligos corresponding to 289 B. melitensis genes was used to identify candidate genes whose transcription is influenced by BlxR. This microarray contains genes encoding the type IV secretion system, flagella, transcriptional regulators, transporters, and proteins involved in outer membrane biogenesis, iron acquisition, and denitrification. RNA was prepared from both B. more...

Organism:

Brucella melitensis

Type:

Expression profiling by array

Platform:

GPL6170

14 Samples

Download data

(Submitter supplied) We report that ancestral zinc-finger-domain transcriptional regulators, previously reported to control virulence/symbiosis, implement a cell cycle (S→G1) transcriptional switch. To unravel how this G1-phase transcriptional program is reinstated during a primitive cell cycle, we first defined G1-specific promoters in the model bacterium Caulobacter crescentus by comparative ChIP-Seq analysis. We then exploited one such promoter as genetic proxy, to identify two conserved developmental regulator paralogs, MucR1/2, that constitute a quadripartite and homeostatic regulatory module directing the switch from S→G1-phase transcription. more...

Organism:

Sinorhizobium fredii; Caulobacter vibrioides

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL18006 GPL18007

11 Samples

Download data: XLS

(Submitter supplied) To investigate the regulatory targets of the RegR virulence regulon of rabbit specific enteropathogenic Escherichia coli strain E22

Organism:

Escherichia coli E22

Type:

Expression profiling by array

Platform:

GPL16094

8 Samples

Download data: TXT

(Submitter supplied) The four-carbon sugar erythritol is an important component of B. melitensis pathogenesis. To determine the transcriptional response to erythritol, B. melitensis strain 16M was grown in the presence of either glucose or erythritol as a sole carbon source.

Organism:

Brucella melitensis bv. 1 str. 16M

Type:

Expression profiling by array

Platform:

GPL15533

4 Samples

Download data: CALLS, PAIR

(Submitter supplied) We report ChIP-seq and RNA-seq analyses of the Brucella abortus LuxR-type transcription factor VjbR

Organism:

Brucella abortus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23154

12 Samples

Download data: BW, NARROWPEAK, TSV

(Submitter supplied) Comparison of the transcriptome profiles of wild-type Brucella abortus and a strain deleted for bab2_0215 (baaR), an IclR-family transcriptional regulator

Organism:

Brucella abortus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24353

6 Samples

Download data: XLSX

(Submitter supplied) Investigation of whole genome gene expression level changes in a B. suis 1330 regA mutant, compared to the wild-type strain. The two-component system RegBA of Brucella suis plays a central role in the control of respiratory systems adapted to oxygen deficiency. The mutant strain is affected in long-term persistence in vitro (this study) and in chronic infection in vivo (Abdou, E et al. 2013, Infect.Immun. more...

Organism:

Brucella suis; Brucella suis 1330

Type:

Expression profiling by array

Platform:

GPL22261

6 Samples

Download data: CALLS, PAIR

(Submitter supplied) Histone-like nucleoid structuring (H-NS) and H-NS-like proteins serve as global gene silencers and work with antagonistic transcriptional activators (counter- silencers) to properly coordinate the expression of virulence genes in pathogenic bacteria. In Brucella, MucR has been proposed as a novel H-NS-like gene silencer, but direct experimental evidence is lacking. Here, we show that MucR serves as an H-NS-like silencer of the Brucella abortus genes encoding the polar autotransporter adhesins BtaE and BmaC, the c-di-GMP-specific phosphodiesterase BpdB, and the quorum-sensing regulator BabR. more...

Organism:

Brucella abortus 2308

Type:

Genome binding/occupancy profiling by high throughput sequencing; Other

Platform:

GPL33491

12 Samples

Download data: CSV, TXT

(Submitter supplied) Introduction: Pantoea stewartii subsp. stewartii is a proteobacterium that causes Stewart’s wilt disease in corn plants. The bacteria form a biofilm in the xylem of infected plants and produce capsule that blocks water transport, eventually causing wilt. At low cell densities, the quorum-sensing (QS) regulatory protein EsaR is known to directly repress expression of esaR itself as well as the capsular synthesis operon transcription regulator, rcsA, and a 2,5-diketogluconate reductase, dkgA. more...

Organism:

Pantoea stewartii

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18688

2 Samples

Download data: XLS

(Submitter supplied) Wild-type Brucella ovis ATCC 25840 requires the addition of 5% CO2 to the atmosphere to grow on either nutrient agar plates or in liquid broth culture. The goal of this study was to measure the transcriptional response of two Brucella ovis strains under high (5%) and low (0.04%) CO2. The two strains assayed were 1) wild-type and 2) a spontaneous mutant that can be cultivated in standard atmospheric levels of CO2 (~0.04%). more...

Organism:

Brucella ovis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26618

10 Samples

Download data: TXT

(Submitter supplied) Microarray analysis determined that 7.82% (244/3334) of Brucella abortus genes were up-regulated and 5.4% (180/3334) were down-regulated in RAW264.7 macrophages, compared to free-living bacteria in TSB

Organism:

Brucella abortus bv. 1 str. 9-941; Brucella abortus str. 2308 A

Type:

Expression profiling by array

Platform:

GPL17087

6 Samples

Download data: TXT

(Submitter supplied) The zur regulon in Neisseria meningitidis was elucidated in the strain MC58 using a zur knockout strain and conditions which activate Zur ( zinc supplementation in the medium)

Organism:

Neisseria meningitidis

Type:

Expression profiling by array

Platform:

GPL8787

11 Samples

Download data: GPR, TXT

(Submitter supplied) Whole genone expression profile comparing wild-type NZ131 to serR deletion mutant, grown in C-medium Mutants and interpretation are described further in the manuscript to be submitted: LaSarre and Federle, 2010. Title: Regulation and Consequence of Serine Catabolism in Streptococcus pyogenes.

Organism:

Streptococcus pyogenes NZ131

Type:

Expression profiling by genome tiling array

Platform:

GPL15576

2 Samples

Download data: CALLS, PAIR, TXT

(Submitter supplied) Transcriptional profiling of A. baumannii ATCC 17978 comparing treated-MMC cultures with non-MMC treated cultures

Organism:

Acinetobacter baumannii ATCC 17978

Type:

Expression profiling by array

Platform:

GPL16729

3 Samples

Download data: TXT

(Submitter supplied) The two-component system CBO0366/CBO0365 was recently demonstrated to have a role in cold tolerance of Group I Clostridium botulinum ATCC 3502. The mechanisms under its control, ultimately resulting in increased sensitivity to low temperature, are unknown. A transcriptomic analysis with DNA microarrays was performed to identify the differences in global gene expression patterns of the wild-type ATCC 3502 and a derivative mutant with insertionally inactivated cbo0365 at 37 °C and 15 °C. more...

Organism:

Clostridium botulinum A str. ATCC 3502

Type:

Expression profiling by array

Platform:

GPL11441

6 Samples

Download data: GPR